CAJ | 학술논문

为表达鸭圆环病毒（DuCV）Cap蛋白，通过Gene Designer 2．0软件对DuCV Cap基因进行分析和密码子优化，构建DuCV Cap基因重组表达质粒 pET-32a-Cap，转化BL21（DH3）株感受态细胞。用终浓度为0．1 mmol/L的IPTG进行诱导表达。SDS-PAGE电泳分析显示Cap融合蛋白的分子质量约为48 ku，表达的重组蛋白占菌体总蛋白的43．1％。Western blot检测显示该融合蛋白可以被针对His标签蛋白的抗体所识别。利用镍离子亲和纯化株对重组蛋白进行了纯化，纯度为92．3％。为进一步建立 DuCV血清学检测方法奠定了基础。
위표체압원배병독（DuCV）Cap단백，통과Gene Designer 2．0연건대DuCV Cap기인진행분석화밀마자우화，구건DuCV Cap기인중조표체질립 pET-32a-Cap，전화BL21（DH3）주감수태세포。용종농도위0．1 mmol/L적IPTG진행유도표체。SDS-PAGE전영분석현시Cap융합단백적분자질량약위48 ku，표체적중조단백점균체총단백적43．1％。Western blot검측현시해융합단백가이피침대His표첨단백적항체소식별。이용얼리자친화순화주대중조단백진행료순화，순도위92．3％。위진일보건립 DuCV혈청학검측방법전정료기출。
For the successful expression of duck circovirus Cap protein,using software Gene Designer 2.0,a codon optimized cap gene was designed in this study.The recombinant expression plasmid pET-32a-cap was transferred to BL21(DH3)competent cells.Following induction by 0.1 mmol/L IPTG ,the bacteria were culture in 37℃ 250 r/min shake for 4 h.The result of SDS-PAGE analysis showed that the fusion Cap pro-tein with molecular bands of about 48 ku was successfully expressed in the form of inclusion body.Western blot proved that the fusion Cap protein could be specifically recognized by the His tag antibody.Finally, recombinant Cap protein was purified by using the NI-ION affinity purification colum,the recombinant pro-tein purity was 92.3%.The Cap protein lays a foundation for the serological detection.