中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
14期
7-9,10
,共4页
子痫前期%大电导钙激活钾通道β1亚基%胎盘%子宫平滑肌%血清
子癇前期%大電導鈣激活鉀通道β1亞基%胎盤%子宮平滑肌%血清
자간전기%대전도개격활갑통도β1아기%태반%자궁평활기%혈청
Preeclampsia%Large conductance Ca2+-activated K+-channelβ1 subunit%Placenta%Uerine smooth muscle tissue%Blood serum
目的：探讨胎盘组织和子宫平滑肌组织中大电导钙激活钾通道β1亚基（KCNMβ1）的表达，及其与子痫前期发病的关系。方法：轻、重度子痫前期组（研究组）和正常孕妇组（对照组）孕妇的胎盘组织及子宫平滑肌组织中KCNMβl mRNA的表达通过荧光定量PCR技术检测。采用ELISA法检测三组孕妇血清KCNMβl浓度变化水平。结果：各研究组中孕妇血清的KCNMβl水平、胎盘组织及子宫平滑肌中KCNMβl mRNA表达水平均明显低于对照组，差异均有统计学意义（P<0.05）；而轻、重度子痫前期组比较差异均无统计学意义（P>0.05）。结论：孕妇血清中KCNMβl表达降低，胎盘组织和子宫平滑肌中KCNMβl mRNA的表达降低与子痫前期的发病密切相关，可能参与了子痫前期的病理生理过程。
目的：探討胎盤組織和子宮平滑肌組織中大電導鈣激活鉀通道β1亞基（KCNMβ1）的錶達，及其與子癇前期髮病的關繫。方法：輕、重度子癇前期組（研究組）和正常孕婦組（對照組）孕婦的胎盤組織及子宮平滑肌組織中KCNMβl mRNA的錶達通過熒光定量PCR技術檢測。採用ELISA法檢測三組孕婦血清KCNMβl濃度變化水平。結果：各研究組中孕婦血清的KCNMβl水平、胎盤組織及子宮平滑肌中KCNMβl mRNA錶達水平均明顯低于對照組，差異均有統計學意義（P<0.05）；而輕、重度子癇前期組比較差異均無統計學意義（P>0.05）。結論：孕婦血清中KCNMβl錶達降低，胎盤組織和子宮平滑肌中KCNMβl mRNA的錶達降低與子癇前期的髮病密切相關，可能參與瞭子癇前期的病理生理過程。
목적：탐토태반조직화자궁평활기조직중대전도개격활갑통도β1아기（KCNMβ1）적표체，급기여자간전기발병적관계。방법：경、중도자간전기조（연구조）화정상잉부조（대조조）잉부적태반조직급자궁평활기조직중KCNMβl mRNA적표체통과형광정량PCR기술검측。채용ELISA법검측삼조잉부혈청KCNMβl농도변화수평。결과：각연구조중잉부혈청적KCNMβl수평、태반조직급자궁평활기중KCNMβl mRNA표체수평균명현저우대조조，차이균유통계학의의（P<0.05）；이경、중도자간전기조비교차이균무통계학의의（P>0.05）。결론：잉부혈청중KCNMβl표체강저，태반조직화자궁평활기중KCNMβl mRNA적표체강저여자간전기적발병밀절상관，가능삼여료자간전기적병리생리과정。
Objective:To investigate the relationship between the pathogenesis of preeclampsia(PE)and the mRNA and protein expression of KCNMβ1 subunit in placenta and uterine smooth muscle tissue.Method:The pregnant placenta tissue and KCNMβl mRNA expression in uterine smooth muscle tissue of the mild and severe preeclampsia group(the study group)and the normal pregnant women(the control group)were detected by fluorescent quantitative PCR technique. The serum KCNMβl concentration level changes of three groups pregnant women were detected by ELISA method. Result:The pregnant women’serum level of KCNM βl,placenta and uterus smooth muscle in KCNM βl mRNA expression levels of the research group were significantly lower than the control group,the difference had statistical significance(P<0.05);there were no significant difference between the mild and severe preeclampsia group(P>0.05). Conclusion:KCNMβl expression in serum of pregnant women is reduced,the placenta tissue and smooth muscle of the uterus KCNMβl mRNA expression in lower closely associated with the onset of preeclampsia,may be involved in the pathophysiological process of preeclampsia.
