河北医学
河北醫學
하북의학
HEBEI MEDICINE
2014年
4期
599-601
,共3页
丹酚酸乙%MCF-7细胞%凋%亡%Caspaed-3蛋白
丹酚痠乙%MCF-7細胞%凋%亡%Caspaed-3蛋白
단분산을%MCF-7세포%조%망%Caspaed-3단백
Salvianolic acid B%MCF-7 cell%Apoptosis%Caspaed-3 protein
目的:观察丹酚酸乙对抑制乳腺癌MCF-7细胞的体外生长及凋亡作用。方法:采用MTT比色法观察丹酚酸乙对体外培养MCF-7细胞增殖抑制作用,并采用流式细胞仪检测丹酚酸乙作用于MCF-7细胞48h后细胞凋亡率和细胞周期变化。结果:丹酚酸乙能明显抑制MCF-7细胞的生长,呈剂量和时间依赖性;流式细胞仪检测结果显示,不同浓度的丹酚酸乙处理MCF-7细胞48 h后,细胞凋亡率和S期细胞的比例逐渐升高。结论:丹酚酸乙对MCF-7细胞具有明显的生长抑制和促凋亡作用。
目的:觀察丹酚痠乙對抑製乳腺癌MCF-7細胞的體外生長及凋亡作用。方法:採用MTT比色法觀察丹酚痠乙對體外培養MCF-7細胞增殖抑製作用,併採用流式細胞儀檢測丹酚痠乙作用于MCF-7細胞48h後細胞凋亡率和細胞週期變化。結果:丹酚痠乙能明顯抑製MCF-7細胞的生長,呈劑量和時間依賴性;流式細胞儀檢測結果顯示,不同濃度的丹酚痠乙處理MCF-7細胞48 h後,細胞凋亡率和S期細胞的比例逐漸升高。結論:丹酚痠乙對MCF-7細胞具有明顯的生長抑製和促凋亡作用。
목적:관찰단분산을대억제유선암MCF-7세포적체외생장급조망작용。방법:채용MTT비색법관찰단분산을대체외배양MCF-7세포증식억제작용,병채용류식세포의검측단분산을작용우MCF-7세포48h후세포조망솔화세포주기변화。결과:단분산을능명현억제MCF-7세포적생장,정제량화시간의뢰성;류식세포의검측결과현시,불동농도적단분산을처리MCF-7세포48 h후,세포조망솔화S기세포적비례축점승고。결론:단분산을대MCF-7세포구유명현적생장억제화촉조망작용。
Objective:To observe the growth inhibiting and apoptosis induced by salvianolic acid B in human breast cancer cell line MCF-7.Method:Cultivation of MCF -7 cell proliferation in vitro was deter-mined, and using flow cytometry instrument for detection apoptosis rate and cell cycle changes Dan phenolic acid b on the MCF -7 cell after 48 hours.Result: Salvianolic acid B can obviously inhibit the growth of MCF-7 cell dependent on dose and time ;Flow cytometry instrument test results showed a gradual increase of the proportion of stage S cells and apoptosis rate after adding different concentrations of acids B .Conclu-sion:Salvianolic acid B has obvious growth inhibition and promoting apoptosis effect on MCF -7 cell.