微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2014年
2期
35-37,41
,共4页
黄芪注射液%肾小管上皮细胞%CD146
黃芪註射液%腎小管上皮細胞%CD146
황기주사액%신소관상피세포%CD146
Astragalus injection%Human renal tubular epithelial cells%CD146
目的：探讨不同剂量黄芪注射液对人肾小管上皮细胞(HK2)黏附分子 CD146表达的干预作用。方法：将传代HK2等分为5组,进行培养处理：正常对照组(LG组,只加5.5mmol/L D-葡萄糖),高糖组(HG组,加入25mmol/L D-葡萄糖),黄芪注射液干预组：在 HG组中加入不同剂量黄芪注射液,加入2μg/ml者为低剂量组(AML组)、加入20μg/ml者为中剂量组(AMM组)、加入200μg/ml 为高剂量组(AMH 组)。观察37℃培养24h、48h、72h 后,各组HK2形态变化、分别收集各组培养细胞,用 Western Blotting检测其CD146蛋白表达。结果：HG组较 LG组 HK2变长,呈梭形,细胞间连接疏松,间隔增大；黄芪注射液干预各组,细胞形态均较 HG组变小变圆,且连接逐渐紧密, AMH 组呈堆积生长。HG组 CD146表达均较 LG组明显增加(P<0.01),干预24h、48h、72h,AMM组、AMH 组与 HG组比较,CD146表达均明显减少(P<0.05或P<0.01),AML 组干预培养72h,CD146的表达亦明显减少(P<0.01)。结论：黄芪注射液改善高糖引起的 HK2形态改变和降低其CD146表达,对防治DN有积极作用。
目的：探討不同劑量黃芪註射液對人腎小管上皮細胞(HK2)黏附分子 CD146錶達的榦預作用。方法：將傳代HK2等分為5組,進行培養處理：正常對照組(LG組,隻加5.5mmol/L D-葡萄糖),高糖組(HG組,加入25mmol/L D-葡萄糖),黃芪註射液榦預組：在 HG組中加入不同劑量黃芪註射液,加入2μg/ml者為低劑量組(AML組)、加入20μg/ml者為中劑量組(AMM組)、加入200μg/ml 為高劑量組(AMH 組)。觀察37℃培養24h、48h、72h 後,各組HK2形態變化、分彆收集各組培養細胞,用 Western Blotting檢測其CD146蛋白錶達。結果：HG組較 LG組 HK2變長,呈梭形,細胞間連接疏鬆,間隔增大；黃芪註射液榦預各組,細胞形態均較 HG組變小變圓,且連接逐漸緊密, AMH 組呈堆積生長。HG組 CD146錶達均較 LG組明顯增加(P<0.01),榦預24h、48h、72h,AMM組、AMH 組與 HG組比較,CD146錶達均明顯減少(P<0.05或P<0.01),AML 組榦預培養72h,CD146的錶達亦明顯減少(P<0.01)。結論：黃芪註射液改善高糖引起的 HK2形態改變和降低其CD146錶達,對防治DN有積極作用。
목적：탐토불동제량황기주사액대인신소관상피세포(HK2)점부분자 CD146표체적간예작용。방법：장전대HK2등분위5조,진행배양처리：정상대조조(LG조,지가5.5mmol/L D-포도당),고당조(HG조,가입25mmol/L D-포도당),황기주사액간예조：재 HG조중가입불동제량황기주사액,가입2μg/ml자위저제량조(AML조)、가입20μg/ml자위중제량조(AMM조)、가입200μg/ml 위고제량조(AMH 조)。관찰37℃배양24h、48h、72h 후,각조HK2형태변화、분별수집각조배양세포,용 Western Blotting검측기CD146단백표체。결과：HG조교 LG조 HK2변장,정사형,세포간련접소송,간격증대；황기주사액간예각조,세포형태균교 HG조변소변원,차련접축점긴밀, AMH 조정퇴적생장。HG조 CD146표체균교 LG조명현증가(P<0.01),간예24h、48h、72h,AMM조、AMH 조여 HG조비교,CD146표체균명현감소(P<0.05혹P<0.01),AML 조간예배양72h,CD146적표체역명현감소(P<0.01)。결론：황기주사액개선고당인기적 HK2형태개변화강저기CD146표체,대방치DN유적겁작용。
Objective:To study the interference on the expression of adhesion molecules CD146 of human renal tubular epi-thelial cells (HK2)by the intervention of different doses of astragalus injection.Method:HK2 were divided into five groups:normal control group (LG group,just add 5.5mmol/L D-glucose),high glucose (HG group,add 25mmol/L D-glucose),astragalus injection intervention groups divided by different concentrations of the HG group on the basis of injection,adding 2μg/ml by low-dose group (AML group),adding 20μg/ml dose group (AMM group), adding 200μg/ml for the high-dose group (AMH group).Cultured at 37 ℃ for 24h,48h,72h,HK2 morphological changes were in each group,and cultured cells were collected at each different time.CD146 protein expression was detected by western bloting.Results:HK2 cultured for 72h in intervention condition,cells of HG group became fusi-form than LG group,the intercellular connections loosed and space increased;in the intervention groups,the cells became round and smaller than LG group,and stacked growth.Compared with LG group,intervented for 24h,48h and 72h,the expression of CD146 of HG group was significantly increased (P<0.01),the AMH and AMM group were also significantly reduced (P<0.01),while the expression of CD146 of AML group was significantly reduced after in-tervention for 72h (P<0.01).Conclusion:High glucose can stimulate an increase in CD146 expression of HK2,different doses of astragalus injection can reduce CD146 expression and connected with the intervention time and dose.
