CAJ | 학술논문

背景：液态芯片技术(Luminex)检测方法是近年来兴起的检测方法，应用液相芯片技术检测抗群体反应抗体，具有灵敏度高，特异性强，检测干扰少，高通量等优点。 　　目的：比较ELISA和Luminex两种方法对肾脏病患者血清标本中群体反应性抗体的检测差异及灵敏度。 　　方法：选取280例肾脏病患者血清标本，同时应用ELISA和Luminex两种方法检测群体反应性抗体阳性率，并运用配对四格表资料的χ2检验进行统计学分析。 　　结果与结论：ELISA法检测群体反应性抗体阳性率为18.9%，Luminex法检测群体反应性抗体阳性率为33.6%。ELISA法检出抗HLA-Ⅰ类抗体和抗HLA-Ⅱ类抗体阳性率分别为12.8%和12.5%；Luminex法检出抗HLA-Ⅰ类抗体和抗HLA-Ⅱ类抗体阳性率分别为25.0%和20.7%。Luminex法的阳性检出率明显高于ELlSA法且Luminex法能准确检测到低浓度抗体。配对四格表资料的χ2检验P<0.01，显示两种方法检测肾脏病患者群体反应性抗体结果差异有显著性意义。结果显示与ELISA法相比，Luminex技术具有更高的灵敏度和准确性，更适合应用于临床检测。
배경：액태심편기술(Luminex)검측방법시근년래흥기적검측방법，응용액상심편기술검측항군체반응항체，구유령민도고，특이성강，검측간우소，고통량등우점。 　　목적：비교ELISA화Luminex량충방법대신장병환자혈청표본중군체반응성항체적검측차이급령민도。 　　방법：선취280례신장병환자혈청표본，동시응용ELISA화Luminex량충방법검측군체반응성항체양성솔，병운용배대사격표자료적χ2검험진행통계학분석。 　　결과여결론：ELISA법검측군체반응성항체양성솔위18.9%，Luminex법검측군체반응성항체양성솔위33.6%。ELISA법검출항HLA-Ⅰ류항체화항HLA-Ⅱ류항체양성솔분별위12.8%화12.5%；Luminex법검출항HLA-Ⅰ류항체화항HLA-Ⅱ류항체양성솔분별위25.0%화20.7%。Luminex법적양성검출솔명현고우ELlSA법차Luminex법능준학검측도저농도항체。배대사격표자료적χ2검험P<0.01，현시량충방법검측신장병환자군체반응성항체결과차이유현저성의의。결과현시여ELISA법상비，Luminex기술구유경고적령민도화준학성，경괄합응용우림상검측。
BACKGROUND:Liquid chip techniques (Luminex) is a recently rising method for detecting anti-panel reactive antibody, which is characterized by high sensitivity, and strong specificity, less interference and high flux. OBJECTIVE:To compare the sensitivity and detection difference of panel reactive antibody in serum of kidney disease patients detected by enzyme-linked immunosorbent assay and Luminex. METHODS:Serum samples of 280 patients with kidney disease were selected. The enzyme-linked immunosorbent assay and Luminex methods were used to measure positive rate of panel reactive antibody. Chi-square test for fourfold table data was utilized for statistical analysis. RESULTS AND CONCLUSION:The positive rates of panel reactive antibody were respectively 18.9%and 33.6%as detected by enzyme-linked immunosorbent assay and Luminex method. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 12.8%and 12.5%, as detected by enzyme-linked immunosorbent assay. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 25.0%and 20.7%, as detected by Luminex method. Positive detection rate was significantly higher in the Luminex group than that in the enzyme-linked immunosorbent assay group. Moreover, Luminex method could precisely detect the low-concentration antibody. Chi-square test for fourfold table data showed P<0.01. Significant differences in the differences of panel reactive antibody of kidney disease patients were detected between the two methods. Results demonstrated that compared with enzyme-linked immunosorbent assay, Luminex method is more sensitive and accurate, and more suitable for clinical detection.