中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
5期
730-735
,共6页
陈昌庆%石炳毅%蔡明%赵豫波%金伯泉%王春艳%韩永%肖漓%周文强%李州利
陳昌慶%石炳毅%蔡明%趙豫波%金伯泉%王春豔%韓永%肖巑%週文彊%李州利
진창경%석병의%채명%조예파%금백천%왕춘염%한영%초리%주문강%리주리
实验动物%组织构建%肾移植%细胞毒性T淋巴细胞相关抗原4%急性排斥反应%尿毒症%共刺激分子%免疫抑制剂
實驗動物%組織構建%腎移植%細胞毒性T淋巴細胞相關抗原4%急性排斥反應%尿毒癥%共刺激分子%免疫抑製劑
실험동물%조직구건%신이식%세포독성T림파세포상관항원4%급성배척반응%뇨독증%공자격분자%면역억제제
transplantation%kidney transplantation%graft rejection%uremia%immunosuppressive agents%flow cytometry
背景：细胞毒性T淋巴细胞相关抗原4是新近发现的共刺激分子，在肿瘤及自身免疫性疾病中研究较多，在肾移植领域缺少研究。<br> 目的：探讨细胞毒性T淋巴细胞相关抗原4在肾移植排斥反应中的作用。<br> 方法：纳入肾移植患者50例，根据移植后肾功能分为2组，急性排斥组20例，移植肾功能稳定组30例。同时选择30例健康查体者作为健康对照组。分别抽取外周静脉血，采用ELISA法及流式细胞术检测观察对象血清及外周血淋巴细胞中的细胞毒性T淋巴细胞相关抗原4水平。<br> 结果与结论：细胞毒性T淋巴细胞相关抗原4在肾移植后急性排斥组、肾功能稳定组及健康对照组血清中的表达水平差异有显著性意义(F=70.0081，P=0.0000)。肾功能稳定组显著低于健康对照组(P=0.0000)，急性排斥组显著低于健康对照组(P=0.0000)，急性排斥组显著低于肾功能稳定组(P=0.0000)。细胞毒性T淋巴细胞相关抗原4在肾移植后急性排斥组、肾功能稳定组及健康对照组淋巴细胞中的表达水平差异无显著性意义(F=1.8656，P=0.1617)。提示细胞毒性T淋巴细胞相关抗原4在肾移植患者发生排斥反应时血清中表达减低，具有一定的相关性，可能参与了排斥反应的发生。
揹景：細胞毒性T淋巴細胞相關抗原4是新近髮現的共刺激分子，在腫瘤及自身免疫性疾病中研究較多，在腎移植領域缺少研究。<br> 目的：探討細胞毒性T淋巴細胞相關抗原4在腎移植排斥反應中的作用。<br> 方法：納入腎移植患者50例，根據移植後腎功能分為2組，急性排斥組20例，移植腎功能穩定組30例。同時選擇30例健康查體者作為健康對照組。分彆抽取外週靜脈血，採用ELISA法及流式細胞術檢測觀察對象血清及外週血淋巴細胞中的細胞毒性T淋巴細胞相關抗原4水平。<br> 結果與結論：細胞毒性T淋巴細胞相關抗原4在腎移植後急性排斥組、腎功能穩定組及健康對照組血清中的錶達水平差異有顯著性意義(F=70.0081，P=0.0000)。腎功能穩定組顯著低于健康對照組(P=0.0000)，急性排斥組顯著低于健康對照組(P=0.0000)，急性排斥組顯著低于腎功能穩定組(P=0.0000)。細胞毒性T淋巴細胞相關抗原4在腎移植後急性排斥組、腎功能穩定組及健康對照組淋巴細胞中的錶達水平差異無顯著性意義(F=1.8656，P=0.1617)。提示細胞毒性T淋巴細胞相關抗原4在腎移植患者髮生排斥反應時血清中錶達減低，具有一定的相關性，可能參與瞭排斥反應的髮生。
배경：세포독성T림파세포상관항원4시신근발현적공자격분자，재종류급자신면역성질병중연구교다，재신이식영역결소연구。<br> 목적：탐토세포독성T림파세포상관항원4재신이식배척반응중적작용。<br> 방법：납입신이식환자50례，근거이식후신공능분위2조，급성배척조20례，이식신공능은정조30례。동시선택30례건강사체자작위건강대조조。분별추취외주정맥혈，채용ELISA법급류식세포술검측관찰대상혈청급외주혈림파세포중적세포독성T림파세포상관항원4수평。<br> 결과여결론：세포독성T림파세포상관항원4재신이식후급성배척조、신공능은정조급건강대조조혈청중적표체수평차이유현저성의의(F=70.0081，P=0.0000)。신공능은정조현저저우건강대조조(P=0.0000)，급성배척조현저저우건강대조조(P=0.0000)，급성배척조현저저우신공능은정조(P=0.0000)。세포독성T림파세포상관항원4재신이식후급성배척조、신공능은정조급건강대조조림파세포중적표체수평차이무현저성의의(F=1.8656，P=0.1617)。제시세포독성T림파세포상관항원4재신이식환자발생배척반응시혈청중표체감저，구유일정적상관성，가능삼여료배척반응적발생。
BACKGROUND:Cytotoxic T lymphocyte-associated antigen 4 is a newly discovered costimulatory molecule. It has been studied more in tumor and autoimmune diseases, less in the field of kidney transplantation. <br> OBJECTIVE:To explore the role of cytotoxic T lymphocyte-associated antigen 4 in acute rejection after renal transplantation. <br> METHODS:Fifty patients undergoing renal transplantation were divided into acute rejection group (20 cases) and stable graft function group (30 cases). Another 30 healthy persons served as control group. Blood samples were extracted from the peripheral blood. Cytotoxic T lymphocyte-associated antigen 4 was detected by enzyme linked immunosorbent assay and flow cytometry. <br> RESULTS AND CONCLUSION:The expression of cytotoxic T lymphocyte-associated antigen 4 in the serum showed significant differences in the acute rejection group, stable graft function group and healthy control group (F=70.008 1, P=0.000 0), but showed no difference in peripheral blood lymphocytes of three groups (F=1.865 6, P=0.161 7). Compared with the healthy control group, the expression levels of cytotoxic T lymphocyte-associated antigen 4 in peripheral blood lymphocytes of acute rejection group and stable graft function group were significantly decreased (P=0.000 0). In addition, the acute rejection group had a lower cytotoxic T lymphocyte-associated antigen 4 expression than the stable graft function group (P=0.000 0). In renal transplant rejection, the expression of cytotoxic T lymphocyte-associated antigen 4 in serum was reduced, showing some correlation with acute rejection after renal transplnatation. Cytotoxic T lymphocyte-associated antigen 4 might be involved in the rejection.
