中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
5期
675-680
,共6页
陈姝%雍春燕%陈恒%初晓%张超%谭程%叶兰%李江山
陳姝%雍春燕%陳恆%初曉%張超%譚程%葉蘭%李江山
진주%옹춘연%진항%초효%장초%담정%협란%리강산
实验动物%组织构建%下丘脑室旁核%胃相关神经元%针刺刺激%国家自然科学基金
實驗動物%組織構建%下丘腦室徬覈%胃相關神經元%針刺刺激%國傢自然科學基金
실험동물%조직구건%하구뇌실방핵%위상관신경원%침자자격%국가자연과학기금
paraventricular hypothalamic nucleus%neurons%gastric dilatation%vagus nerve%acupuncture points
背景：既往有关针刺对胃功能调节的神经机制研究多以外周神经为主，涉及中枢，尤其是高位中枢的研究较少。<br> 目的：探讨针刺“内关”、“足三里”等穴位对下丘脑室旁核胃相关神经元作用的影响。<br> 方法：运用微电极细胞外记录技术，在大鼠胃扩张的基础上，找出室旁核胃相关神经元，予以手针刺激“内关”、“足三里”等穴位各30 s，观察其对室旁核胃相关神经元的影响。<br> 结果与结论：60只大鼠共记录到109个下丘脑室旁核神经元放电，109个下丘脑室旁核神经元中与胃扩张相关的神经元有56个，56个胃扩张相关神经元中，对针刺足三里、内关、脾俞、胃俞有反应的神经元个数分别是44，47，29，33，有反应的神经元出现的比例分别是78.57%，83.47%，51.79%，58.93%。结果表明下丘脑室旁核中存在同时对胃扩张刺激和针刺刺激起反应的躯体内脏汇聚神经元，且参与针刺对胃功能的调节作用。
揹景：既往有關針刺對胃功能調節的神經機製研究多以外週神經為主，涉及中樞，尤其是高位中樞的研究較少。<br> 目的：探討針刺“內關”、“足三裏”等穴位對下丘腦室徬覈胃相關神經元作用的影響。<br> 方法：運用微電極細胞外記錄技術，在大鼠胃擴張的基礎上，找齣室徬覈胃相關神經元，予以手針刺激“內關”、“足三裏”等穴位各30 s，觀察其對室徬覈胃相關神經元的影響。<br> 結果與結論：60隻大鼠共記錄到109箇下丘腦室徬覈神經元放電，109箇下丘腦室徬覈神經元中與胃擴張相關的神經元有56箇，56箇胃擴張相關神經元中，對針刺足三裏、內關、脾俞、胃俞有反應的神經元箇數分彆是44，47，29，33，有反應的神經元齣現的比例分彆是78.57%，83.47%，51.79%，58.93%。結果錶明下丘腦室徬覈中存在同時對胃擴張刺激和針刺刺激起反應的軀體內髒彙聚神經元，且參與針刺對胃功能的調節作用。
배경：기왕유관침자대위공능조절적신경궤제연구다이외주신경위주，섭급중추，우기시고위중추적연구교소。<br> 목적：탐토침자“내관”、“족삼리”등혈위대하구뇌실방핵위상관신경원작용적영향。<br> 방법：운용미전겁세포외기록기술，재대서위확장적기출상，조출실방핵위상관신경원，여이수침자격“내관”、“족삼리”등혈위각30 s，관찰기대실방핵위상관신경원적영향。<br> 결과여결론：60지대서공기록도109개하구뇌실방핵신경원방전，109개하구뇌실방핵신경원중여위확장상관적신경원유56개，56개위확장상관신경원중，대침자족삼리、내관、비유、위유유반응적신경원개수분별시44，47，29，33，유반응적신경원출현적비례분별시78.57%，83.47%，51.79%，58.93%。결과표명하구뇌실방핵중존재동시대위확장자격화침자자격기반응적구체내장회취신경원，차삼여침자대위공능적조절작용。
BACKGROUND:Previous studies concerning neuromechanism of acupuncture for regulating gastric function mainly focused on peripheral nerve, involving in central nervous system. In particular, there are few studies addressing higher central nervous system. <br> OBJECTIVE:To explore the effects of acupuncture at Neiguan (PC6) and Zusanli (ST36) on gastric-related neurons in the hypothalamic paraventricular nucleus. <br> METHODS:Using microelectrode extracellular recording technique, gastric related neurons in the paraventricular nucleus were found in gastric distension rats. Stimulation was conducted for 30 seconds by hand acupuncture at Neiguan and Zusanli. The effects of acupuncture on gastric-related neurons in the paraventricular nucleus were observed. <br> RESULTS AND CONCLUSION:The discharge of 109 neurons in the hypothalamic paraventricular nucleus of 60 rats was recorded. A total of 56 gastric-related neurons of the 109 neurons were observed. The number of neurons response to acupunctures at Zusanli, Neiguan, Pishu (BL20) and Weishu (BL21) were respectively 44, 47, 29, and 33, with the reaction proportion of 78.57%, 83.47%, 51.79%and 58.93%. Results suggested that there exist gastric distension stimulation and acupuncture stimulations from neurons soma to visceral reaction in the hypothalamic paraventricular nucleus, which simultaneously participates in the regulation of acupuncture on the gastric function.
