中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
5期
669-674
,共6页
张强%王洪玲%丁弦%夏晨蕾%刘丽娟%王爽玉%李建平%贺苗%孙文娜%阎潇%刘文%张月%姚如永%袁晓
張彊%王洪玲%丁絃%夏晨蕾%劉麗娟%王爽玉%李建平%賀苗%孫文娜%閻瀟%劉文%張月%姚如永%袁曉
장강%왕홍령%정현%하신뢰%류려연%왕상옥%리건평%하묘%손문나%염소%류문%장월%요여영%원효
实验动物%组织构建%GRP78%周期性张应力%内质网应激%L6%成肌细胞%功能矫形%国家自然科学基金
實驗動物%組織構建%GRP78%週期性張應力%內質網應激%L6%成肌細胞%功能矯形%國傢自然科學基金
실험동물%조직구건%GRP78%주기성장응력%내질망응격%L6%성기세포%공능교형%국가자연과학기금
endoplasmic reticulum%myoblasts%apoptosis%immunoglobulin heavy chains%mechanics
背景：内质网应激参与多种疾病的发生发展过程，如动脉粥样硬化、糖尿病、阿尔茨海默病，GRP78是内质网应激的标志蛋白，GRP78的表达水平反映内质网应激的程度。<br> 目的：研究周期性张应力对 L6大鼠成肌细胞 GRP78表达的影响，以明确周期性张应力与内质网应激的关系。<br> 方法：在成功构建L6大鼠成肌细胞体外培养力学刺激模型的基础上，采用RT-PCR和Western blot法分析周期性张应力对GRP78 mRNA和蛋白表达的影响。加力组分别给予1，6，12，24 h的力学刺激，加载频率为10 cycles/min，拉伸变形幅度为15%。对照组与实验组在同时种板，不给予力刺激。<br> 结果与结论：GRP78 mRNA随着加力时间的延长呈一致上升趋势，与对照组相比，差异均有显著性意义(P<0.05)；GRP78蛋白在加力1 h后，蛋白表达量开始升高，加力6 h后蛋白表达量显著升高，到24 h达到峰值，与对照组相比，差异均有显著性意义(P<0.05)。由以上结果得出，在一定时间范围内，周期性张应力可引起内质网应激，并且随着时间的延长逐渐增强；持续的应力刺激，可引起严重的内质网应激导致成肌细胞凋亡。
揹景：內質網應激參與多種疾病的髮生髮展過程，如動脈粥樣硬化、糖尿病、阿爾茨海默病，GRP78是內質網應激的標誌蛋白，GRP78的錶達水平反映內質網應激的程度。<br> 目的：研究週期性張應力對 L6大鼠成肌細胞 GRP78錶達的影響，以明確週期性張應力與內質網應激的關繫。<br> 方法：在成功構建L6大鼠成肌細胞體外培養力學刺激模型的基礎上，採用RT-PCR和Western blot法分析週期性張應力對GRP78 mRNA和蛋白錶達的影響。加力組分彆給予1，6，12，24 h的力學刺激，加載頻率為10 cycles/min，拉伸變形幅度為15%。對照組與實驗組在同時種闆，不給予力刺激。<br> 結果與結論：GRP78 mRNA隨著加力時間的延長呈一緻上升趨勢，與對照組相比，差異均有顯著性意義(P<0.05)；GRP78蛋白在加力1 h後，蛋白錶達量開始升高，加力6 h後蛋白錶達量顯著升高，到24 h達到峰值，與對照組相比，差異均有顯著性意義(P<0.05)。由以上結果得齣，在一定時間範圍內，週期性張應力可引起內質網應激，併且隨著時間的延長逐漸增彊；持續的應力刺激，可引起嚴重的內質網應激導緻成肌細胞凋亡。
배경：내질망응격삼여다충질병적발생발전과정，여동맥죽양경화、당뇨병、아이자해묵병，GRP78시내질망응격적표지단백，GRP78적표체수평반영내질망응격적정도。<br> 목적：연구주기성장응력대 L6대서성기세포 GRP78표체적영향，이명학주기성장응력여내질망응격적관계。<br> 방법：재성공구건L6대서성기세포체외배양역학자격모형적기출상，채용RT-PCR화Western blot법분석주기성장응력대GRP78 mRNA화단백표체적영향。가력조분별급여1，6，12，24 h적역학자격，가재빈솔위10 cycles/min，랍신변형폭도위15%。대조조여실험조재동시충판，불급여력자격。<br> 결과여결론：GRP78 mRNA수착가력시간적연장정일치상승추세，여대조조상비，차이균유현저성의의(P<0.05)；GRP78단백재가력1 h후，단백표체량개시승고，가력6 h후단백표체량현저승고，도24 h체도봉치，여대조조상비，차이균유현저성의의(P<0.05)。유이상결과득출，재일정시간범위내，주기성장응력가인기내질망응격，병차수착시간적연장축점증강；지속적응력자격，가인기엄중적내질망응격도치성기세포조망。
BACKGROUND:Endoplasmic reticulum stress participates in the occurrence and development of many diseases, such as atherosclerosis, diabetes, and Alzheimer’s disease. GRP78 is a marker of endoplasmic reticulum stress. The expression of GRP78 reflects the degree of endoplasmic reticulum stress. <br> OBJECTIVE:To investigate the effect of cyclic stretch on GRP78 expression of L6 rat myoblasts, and to identify the relationship between cyclic stretch and endoplasmic reticulum stress. <br> METHODS:In vitro culture-tensile stimulation models of myoblasts of L6 rats were established successful y. The expression of GRP78 of myoblasts exposed to cyclic stretch was determined by reverse transcription-PCR and western blot assay. Stretch groups were subjected to 15%surface elongation at a frequency of 10 cycles per minute, over a period of 1, 6, 12 and 24 hours. cells were simultaneously seeded on a plate in the control and experimental groups with no stimulation. <br> RESULTS AND CONCLUSION:The expression of GRP78 mRNA was continuously elevated over time after stretched treatment, and significant differences were detected as compared with the control group (P<0.05). GRP78 protein expression began to increase at 1 hour after stretched treatment, was significantly increased at 6 hours, peaked at 24 hours, and significant differences were visible as compared with the control group (P<0.05). In conclusion, cyclic stretch induced the occurrence of endoplasmic reticulum stress, which was enhanced with prolonged time. However, prolonged stretch caused severe endoplasmic reticulum stress and leaded to apoptosis of myoblasts.
