CAJ | 학술논문

目的：利用原核表达并纯化的人细小病毒B19（HPV B19）结构蛋白VP1初步建立HPV B19 IgM酶联免疫吸附试验（ELISA）的检测方法。方法以纯化的重组蛋白包被酶标板，建立IgM 间接ELISA检测方法，确定Cut-off值，并进行初步的应用。结果建立间接 ELISA方法Cut-off值为0．25，灵敏度为84．60％，特异性为99．70％，与对照试剂盒检测结果符合率为99．47％；用自产试剂盒检测115份孕妇和1700份健康献血志愿者血清中B19 IgM抗体，阳性率分别为12．17％和1．59％。结论通过包被 HPV B19-VP1蛋白建立的间接ELISA检测方法可用于 HPV B19早期感染或急性感染的辅助诊断。
목적：이용원핵표체병순화적인세소병독B19（HPV B19）결구단백VP1초보건립HPV B19 IgM매련면역흡부시험（ELISA）적검측방법。방법이순화적중조단백포피매표판，건립IgM 간접ELISA검측방법，학정Cut-off치，병진행초보적응용。결과건립간접 ELISA방법Cut-off치위0．25，령민도위84．60％，특이성위99．70％，여대조시제합검측결과부합솔위99．47％；용자산시제합검측115빈잉부화1700빈건강헌혈지원자혈청중B19 IgM항체，양성솔분별위12．17％화1．59％。결론통과포피 HPV B19-VP1단백건립적간접ELISA검측방법가용우 HPV B19조기감염혹급성감염적보조진단。
Objective Investigated prokaryotic expression and purification of structural protein VP 1 of human parvovirus B19(HPV B19) ,and detected HPV infection by an IgM-ELISA method .Methods We established and preliminarily applied an indirect ELISA method detecting IgM where the purified recombinant protein (HPV B19-VP1) coated in microtiter plate as immunosorbent .Results In this study ,we observed that the Cut-off value ,sensi-tivity ,and specificity of the IgM-ELISA established were 0 .25 ,84 .6% ,and 99 .7% respectively .The coincidence rate of determination results by the home made kit and control kit was 99 .47% .A total of 1700 serum samples from healthy donors and 115 serum samples from pregnant women were with HPV B19 IgM determined by the IgM-ELISA established ,in which the positive rates of B19 IgM were 1 .59% and 12 .17% respectively .Conclusion Our results suggested that the indirect ELISE-IgM ,in which HPV B19-VP1 were coated ,may be an appropriate assisted method to diagnose the early or acute phase of HPV B19 infection .