中国现代医药杂志
中國現代醫藥雜誌
중국현대의약잡지
MODERN MEDICINE JOURNAL OF CHINA
2014年
4期
27-31
,共5页
FTY720%创伤性休克%急性肺损伤%缺血再灌注损伤%存活率
FTY720%創傷性休剋%急性肺損傷%缺血再灌註損傷%存活率
FTY720%창상성휴극%급성폐손상%결혈재관주손상%존활솔
FTY720%Traumatic shock%ALI%Reperfusion injury%Survival rate
目的:观察FTY720对创伤性休克兔急性肺损伤的保护作用。方法采用Lamson′s法建立创伤性休克动物模型。取72只家兔随机分为6组,即假休克组( CON )、乳酸钠林格氏液( LR )复苏组、乳酸钠林格氏液联合地塞米松(LR/DXM)复苏组、乳酸钠林格氏液联合 FTY720(LR/FTY720)复苏组,其中 FTY720分为低剂量(FTY720-L)、中剂量(FTY20-M)、高剂量(FTY20-H),剂量分别为0.1mg/kg、0.3mg/kg、0.9mg/kg。复苏过程中监测血流动力学变化,复苏后2h测血气分析。采用ELISA法检测血浆中及支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中TNF-α、IL-6、IL-10的变化。用BCA法检测BALF中蛋白含量。复苏后2h各组随机处死6只动物,用HE染色观察肺组织的病理变化。各组剩余动物(每组6只)给予正常饮食观察24h、48h、72h 的存活率。结果与LR组比较,各组间差异有统计学意义(P<0.05);与DXM组相比,各组间差异有统计学意义(P<0.05);FYT720组内比较差异有统计学意义(P<0.05),即应用FYT720-M复苏组肺组织的病理损害明显减轻,血浆及BALF中TNF-α、IL-6含量明显降低,IL-10含量明显提高,72h存活率明显提高。结论 FTY720对创伤性休克兔所致急性肺损伤有保护作用,并且提高了创伤后的存活率。
目的:觀察FTY720對創傷性休剋兔急性肺損傷的保護作用。方法採用Lamson′s法建立創傷性休剋動物模型。取72隻傢兔隨機分為6組,即假休剋組( CON )、乳痠鈉林格氏液( LR )複囌組、乳痠鈉林格氏液聯閤地塞米鬆(LR/DXM)複囌組、乳痠鈉林格氏液聯閤 FTY720(LR/FTY720)複囌組,其中 FTY720分為低劑量(FTY720-L)、中劑量(FTY20-M)、高劑量(FTY20-H),劑量分彆為0.1mg/kg、0.3mg/kg、0.9mg/kg。複囌過程中鑑測血流動力學變化,複囌後2h測血氣分析。採用ELISA法檢測血漿中及支氣管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中TNF-α、IL-6、IL-10的變化。用BCA法檢測BALF中蛋白含量。複囌後2h各組隨機處死6隻動物,用HE染色觀察肺組織的病理變化。各組剩餘動物(每組6隻)給予正常飲食觀察24h、48h、72h 的存活率。結果與LR組比較,各組間差異有統計學意義(P<0.05);與DXM組相比,各組間差異有統計學意義(P<0.05);FYT720組內比較差異有統計學意義(P<0.05),即應用FYT720-M複囌組肺組織的病理損害明顯減輕,血漿及BALF中TNF-α、IL-6含量明顯降低,IL-10含量明顯提高,72h存活率明顯提高。結論 FTY720對創傷性休剋兔所緻急性肺損傷有保護作用,併且提高瞭創傷後的存活率。
목적:관찰FTY720대창상성휴극토급성폐손상적보호작용。방법채용Lamson′s법건립창상성휴극동물모형。취72지가토수궤분위6조,즉가휴극조( CON )、유산납림격씨액( LR )복소조、유산납림격씨액연합지새미송(LR/DXM)복소조、유산납림격씨액연합 FTY720(LR/FTY720)복소조,기중 FTY720분위저제량(FTY720-L)、중제량(FTY20-M)、고제량(FTY20-H),제량분별위0.1mg/kg、0.3mg/kg、0.9mg/kg。복소과정중감측혈류동역학변화,복소후2h측혈기분석。채용ELISA법검측혈장중급지기관폐포관세액(bronchoalveolar lavage fluid,BALF)중TNF-α、IL-6、IL-10적변화。용BCA법검측BALF중단백함량。복소후2h각조수궤처사6지동물,용HE염색관찰폐조직적병리변화。각조잉여동물(매조6지)급여정상음식관찰24h、48h、72h 적존활솔。결과여LR조비교,각조간차이유통계학의의(P<0.05);여DXM조상비,각조간차이유통계학의의(P<0.05);FYT720조내비교차이유통계학의의(P<0.05),즉응용FYT720-M복소조폐조직적병리손해명현감경,혈장급BALF중TNF-α、IL-6함량명현강저,IL-10함량명현제고,72h존활솔명현제고。결론 FTY720대창상성휴극토소치급성폐손상유보호작용,병차제고료창상후적존활솔。
Objective To observe the protective effect of FTY720 on acute lung injury (ALI) in a rabbit model of trau-matic shock. Methods The traumatic shock model was established from improved Lamson's method. 72 rabbits were randomly divided into 6 groups (n=12 each):group CON,group LR,group LR/DXM,group LR/FTY720 (0.1mg/kg,0.3mg/kg,0.9mg/kg). Observed the change of hemodynamic in the process of fluid resuscitation. 2 hours after resuscitation , the blood gas analysis was measured. Enzyme immunosorbent test was used to detect the IL-6,IL-10,TNF-α level in blood and bronchoalveolar lavage fluid(BALF). Measured the total protein in BALF by BCA method. 2 hours after resuscitation, the 6 rabbits were randomly killed and observed the pathology change of lung organization by HE dye. The left rabbits (n=6,each group) fed normal food was ob-served for 24h,48h,72h to determine survival rate. Results Compared with other groups the damage of lung organization was lessened significantly and the expression of IL-6,IL-10,TNF-αdecreased in FTY720-M group. But the IL-10 and survival rate increased in FTY720-M group. Conclusion FTY720 has protective effect on ALI for Traumatic shock rabbit, and it can in-crease the survival rate after trauma.