CAJ | 학술논문

万方数据

中国医学创新 중국의학창신
MEDICAL INNOVATION OF CHINA
2014年 6期 19-20 ,共2页

石磊%杨伟明%赵洪远%骆礼波%贺新媛%任宇%苏晓玥%刘勤%陈梦婷%霍金龙

석뢰%양위명%조홍원%락례파%하신원%임우%소효모%류근%진몽정%곽금룡

大肠癌%肿瘤浸润淋巴细胞%石蜡包埋%流式细胞术大腸癌%腫瘤浸潤淋巴細胞%石蠟包埋%流式細胞術
대장암%종류침윤림파세포%석사포매%류식세포술
Colorectal carcinoma%Tumor infiltrating lymphocytes%Paraffin-embedded%Flow cytometry

探讨石蜡标本中大肠癌肿瘤浸润淋巴细胞亚群单细胞悬液样本的制备方法。石蜡标本经切片、脱蜡、水化、消化、过滤、收集细胞等步骤制成单细胞悬液，滴加单克隆抗体，通过流式细胞仪检测，最后进行淋巴细胞亚群细胞分型获取及读数。
탐토석사표본중대장암종류침윤림파세포아군단세포현액양본적제비방법。석사표본경절편、탈사、수화、소화、과려、수집세포등보취제성단세포현액，적가단극륭항체，통과류식세포의검측，최후진행림파세포아군세포분형획취급독수。
To explore the ways of making the FCM checking tumor infiltrating lymphocytes in colorectal carcinoma into single cell suspension. The collection was made into single cell suspension by sliced,deparaffinage,hydration,digestion,filtration and cells collection,adding monoclonal antibody and checking by flow cytometer,getting lymphocyte subpopulations and fetching the dates.