中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
6期
3-5
,共3页
王广新%马衍辉%周荣双%于磊%伊长英%任士锋
王廣新%馬衍輝%週榮雙%于磊%伊長英%任士鋒
왕엄신%마연휘%주영쌍%우뢰%이장영%임사봉
缺氧诱导因子1α%多药耐药基因1%难治性癫痫
缺氧誘導因子1α%多藥耐藥基因1%難治性癲癇
결양유도인자1α%다약내약기인1%난치성전간
Hypoxia induced factor 1α%Multidrug resistance gene 1%Refractory epilepsy
目的：探讨难治性癫痫患者脑内缺氧诱导因子-1α（HIF-1α）与多药耐药基因1（MDR1）的表达情况、相关性及其意义。方法：收集9例难治性癫痫组手术切除脑组织标本及7例对照组脑组织标本，采用免疫组织化学染色方法检测HIF-1α和MDR1的表达情况，并分析HIF-1α与MDR1表达阳性率的相关性。结果：HIF-1α在难治性癫痫组脑组织中的阳性表达率77.8%（7/9）明显高于对照组的14.3%（1/7）；MDR1在难治性癫痫组脑组织中的阳性表达率66.7%（6/9）明显高于对照组的14.3%（1/7），比较差异均有统计学意义（P<0.05）；难治性癫痫组脑组织中HIF-1α与MDR1的表达呈正相关（r=0.76，P<0.05）。结论：难治性癫痫患者脑内HIF-1α和MDR1表达升高，且两者呈正相关。HIF-1α可能通过调控MDR1基因表达而参与难治性癫痫的形成。
目的：探討難治性癲癇患者腦內缺氧誘導因子-1α（HIF-1α）與多藥耐藥基因1（MDR1）的錶達情況、相關性及其意義。方法：收集9例難治性癲癇組手術切除腦組織標本及7例對照組腦組織標本，採用免疫組織化學染色方法檢測HIF-1α和MDR1的錶達情況，併分析HIF-1α與MDR1錶達暘性率的相關性。結果：HIF-1α在難治性癲癇組腦組織中的暘性錶達率77.8%（7/9）明顯高于對照組的14.3%（1/7）；MDR1在難治性癲癇組腦組織中的暘性錶達率66.7%（6/9）明顯高于對照組的14.3%（1/7），比較差異均有統計學意義（P<0.05）；難治性癲癇組腦組織中HIF-1α與MDR1的錶達呈正相關（r=0.76，P<0.05）。結論：難治性癲癇患者腦內HIF-1α和MDR1錶達升高，且兩者呈正相關。HIF-1α可能通過調控MDR1基因錶達而參與難治性癲癇的形成。
목적：탐토난치성전간환자뇌내결양유도인자-1α（HIF-1α）여다약내약기인1（MDR1）적표체정황、상관성급기의의。방법：수집9례난치성전간조수술절제뇌조직표본급7례대조조뇌조직표본，채용면역조직화학염색방법검측HIF-1α화MDR1적표체정황，병분석HIF-1α여MDR1표체양성솔적상관성。결과：HIF-1α재난치성전간조뇌조직중적양성표체솔77.8%（7/9）명현고우대조조적14.3%（1/7）；MDR1재난치성전간조뇌조직중적양성표체솔66.7%（6/9）명현고우대조조적14.3%（1/7），비교차이균유통계학의의（P<0.05）；난치성전간조뇌조직중HIF-1α여MDR1적표체정정상관（r=0.76，P<0.05）。결론：난치성전간환자뇌내HIF-1α화MDR1표체승고，차량자정정상관。HIF-1α가능통과조공MDR1기인표체이삼여난치성전간적형성。
Objective:To explore expressions and correlation of hypoxia induced factor 1α(HIF-1α)and multidrug resistance gene 1(MDR1) in the brain tissues of patients with refractory epilepsy.Method:Brain tissues were collected from 9 patients with refractory epilepsy and 7 cases of the control group.The expression of HIF-1αand MDR1 were examined by immunohistochemistry,and the correlation between the positive expression rates of HIF-1αand MDR1 were analyzed.Result:The positive expression rate of HIF-1αin refractory epilepsy group(77.8%)was higher significantly than that in control group(14.3%),the positive expression rate of MDR1 in refractory epilepsy group(66.7%)was higher significantly than that in control group(14.3%),the differences were statistically significant(P<0.05).The expression of HIF-1αwas positively correlated with the expression of MDR1(r=0.76,P<0.05).Conclusion:HIF-1αand MDR1 are over expressed in the brain of refractory epilepsy,and there is a positive correlation. HIF-1αmay be involved in the pathogenesis of refractory epilepsy by regulating the expression of MDR1 gene.
