CAJ | 학술논문

目的：以大鼠为动物模型探讨17β-雌二醇对于早产儿视网膜病变（ retinopathy of prematurity ， ROP ）的预防作用并探讨其机制。 　　方法：选取7日龄SD大鼠80只随机分为正常对照组、单纯高氧组、雌二醇干预组以及溶媒干预组，单纯高氧组于氧浓度75％的容器内饲养5d，再置正常空气下饲养至17日龄，雌二醇干预组饲养方法同单纯高氧组，在大鼠7～17日龄间每日皮下注射17β-雌二醇（ E2，植物油稀释，配制浓度为2μg/μL），每只大鼠0．5μL/d，溶媒干预组饲养方法与雌二醇组相同，药物改为相同体积植物油。通过HE染色、视网膜铺片观测血管改变和增生情况，免疫组化及RT-PCR检测VEGF的表达。 　　结果：雌二醇干预组突破内界膜的内皮细胞较单纯高氧组及溶媒干预组明显减少（P＜0．05）；视网膜铺片可见雌二醇干预组较之单纯高氧组血管网有不同程度的改善，血管结构清晰，形态基本正常；免疫组织化学染色以及 RT-PCR结果显示：VEGF在雌二醇干预组的表达较之单纯高氧组及溶媒干预组降低，但仍高于正常组。 　　结论：17β-雌二醇可在一定程度上预防ROP新生血管的产生，机制可能与抑制VEGF的表达相关。
목적：이대서위동물모형탐토17β-자이순대우조산인시망막병변（ retinopathy of prematurity ， ROP ）적예방작용병탐토기궤제。 　　방법：선취7일령SD대서80지수궤분위정상대조조、단순고양조、자이순간예조이급용매간예조，단순고양조우양농도75％적용기내사양5d，재치정상공기하사양지17일령，자이순간예조사양방법동단순고양조，재대서7～17일령간매일피하주사17β-자이순（ E2，식물유희석，배제농도위2μg/μL），매지대서0．5μL/d，용매간예조사양방법여자이순조상동，약물개위상동체적식물유。통과HE염색、시망막포편관측혈관개변화증생정황，면역조화급RT-PCR검측VEGF적표체。 　　결과：자이순간예조돌파내계막적내피세포교단순고양조급용매간예조명현감소（P＜0．05）；시망막포편가견자이순간예조교지단순고양조혈관망유불동정도적개선，혈관결구청석，형태기본정상；면역조직화학염색이급 RT-PCR결과현시：VEGF재자이순간예조적표체교지단순고양조급용매간예조강저，단잉고우정상조。 　　결론：17β-자이순가재일정정도상예방ROP신생혈관적산생，궤제가능여억제VEGF적표체상관。
AIM:To explore the effects and mechanisms of 17β-estradiol on retinopathy of prematurity in rats. METHODS: Eighty 7-day-old SD rats were randomly divided into normol group, high oxygen group, 17β-estradiol treatment group and vegetable oil treatment group.Rats of the high oxygen group were put into the environment exposed to 75%oxygen for 5d and backed to room air for another 5d to establish the oxygen-induced retinopathy model.The treatment group was given 17β-estradiol by injection with the dose of 0.5uL/rat/d (2μg/μL) before exposed to 75% oxygen. The vegetable oil treatment group is similar to 17β-estradiol treatment group but the medicine changed to vegetable oil. Counting the endotheliocyte nuclei of new vessels which extended from retina to vitreous body in the tissue-slice of HE staining, and investigate the change of retinal blood vessels by using methods of retina flat -mount. The expression of VEGF in retina by immunohistochemistry and reverse transcription-polymerase chain reaction ( RT-PCR) analysis. RESULTS: Rats treated with E2 showed less neovascularization than high oxygen group and oxygen-exposed rats treated with vegetable oil ( P <0.05 ) . Adenosine diphosphate-ase ( ADP ase) stained retina flat-mount showed that: less free-vascular areas and new blood vessels were seen in the 17β-estradiol treatment group compared with the high oxygen group. The expression of VEGF in 17β-estradiol treatment group lower than high oxygen group and vegetable oil treatment group, but higher than normal group in immunohistochemistry and RT-PCR analysis. CONCLUSION:17β-estradiol has prevention effects in retinal neovascularization and the mechanism may involve in its interaction with VEGF.