国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
3期
402-406
,共5页
糖尿病视网膜病变%视网膜神经节细胞%凋亡%钙离子浓度
糖尿病視網膜病變%視網膜神經節細胞%凋亡%鈣離子濃度
당뇨병시망막병변%시망막신경절세포%조망%개리자농도
diabetic retinopathy%retinal ganglion cells%apoptosis%calcium ion concentration
目的：探讨糖尿病大鼠模型早期视网膜神经节细胞（ retinal ganglion cells ，RGCs）凋亡的机制。<br> 方法：SD大鼠60只，随机分为对照组（ CON ）及糖尿病组（ DM），糖尿病组一次性腹腔注射1％STZ诱发糖尿病鼠模型，两组于第4，8，12 wk分别行HE、透射电镜及TUNEL法检测RGCs的凋亡情况，并应用激光共聚焦显微镜检测RGCs内钙离子浓度的变化。<br> 结果：糖尿病组第8 wk开始出现RGCs数量减少、细胞排列紊乱的病理改变，第12 wk更为明显。糖尿病组透射电镜下可见第4 wk 时 RGCs 出现线粒体肿胀；第8 wk 时RGCs内肿胀的线粒体更为明显、数目增多，染色质边集于核膜周边，部分细胞体积缩小、细胞器减少；第12 wk时出现RGCs体积变小，甚至出现细胞核断裂。 TUNEL阳性RGCs最早于糖尿病组第4wk时出现，随病程延长凋亡的阳性细胞逐渐增多，凋亡指数与同期对照组相比，差异有统计学意义（P＜0．01）。糖尿病组第8，12wk 时RGCs内钙离子浓度明显高于同期对照组，差异显著（ P＜0．01）；糖尿病组第8 wk与第12 wk比较，升高差异亦有统计学意义（P＜0．05）。<br> 结论：糖尿病早期出现了视网膜神经节细胞的凋亡，其机制可能与细胞内钙离子浓度升高有关。
目的：探討糖尿病大鼠模型早期視網膜神經節細胞（ retinal ganglion cells ，RGCs）凋亡的機製。<br> 方法：SD大鼠60隻，隨機分為對照組（ CON ）及糖尿病組（ DM），糖尿病組一次性腹腔註射1％STZ誘髮糖尿病鼠模型，兩組于第4，8，12 wk分彆行HE、透射電鏡及TUNEL法檢測RGCs的凋亡情況，併應用激光共聚焦顯微鏡檢測RGCs內鈣離子濃度的變化。<br> 結果：糖尿病組第8 wk開始齣現RGCs數量減少、細胞排列紊亂的病理改變，第12 wk更為明顯。糖尿病組透射電鏡下可見第4 wk 時 RGCs 齣現線粒體腫脹；第8 wk 時RGCs內腫脹的線粒體更為明顯、數目增多，染色質邊集于覈膜週邊，部分細胞體積縮小、細胞器減少；第12 wk時齣現RGCs體積變小，甚至齣現細胞覈斷裂。 TUNEL暘性RGCs最早于糖尿病組第4wk時齣現，隨病程延長凋亡的暘性細胞逐漸增多，凋亡指數與同期對照組相比，差異有統計學意義（P＜0．01）。糖尿病組第8，12wk 時RGCs內鈣離子濃度明顯高于同期對照組，差異顯著（ P＜0．01）；糖尿病組第8 wk與第12 wk比較，升高差異亦有統計學意義（P＜0．05）。<br> 結論：糖尿病早期齣現瞭視網膜神經節細胞的凋亡，其機製可能與細胞內鈣離子濃度升高有關。
목적：탐토당뇨병대서모형조기시망막신경절세포（ retinal ganglion cells ，RGCs）조망적궤제。<br> 방법：SD대서60지，수궤분위대조조（ CON ）급당뇨병조（ DM），당뇨병조일차성복강주사1％STZ유발당뇨병서모형，량조우제4，8，12 wk분별행HE、투사전경급TUNEL법검측RGCs적조망정황，병응용격광공취초현미경검측RGCs내개리자농도적변화。<br> 결과：당뇨병조제8 wk개시출현RGCs수량감소、세포배렬문란적병리개변，제12 wk경위명현。당뇨병조투사전경하가견제4 wk 시 RGCs 출현선립체종창；제8 wk 시RGCs내종창적선립체경위명현、수목증다，염색질변집우핵막주변，부분세포체적축소、세포기감소；제12 wk시출현RGCs체적변소，심지출현세포핵단렬。 TUNEL양성RGCs최조우당뇨병조제4wk시출현，수병정연장조망적양성세포축점증다，조망지수여동기대조조상비，차이유통계학의의（P＜0．01）。당뇨병조제8，12wk 시RGCs내개리자농도명현고우동기대조조，차이현저（ P＜0．01）；당뇨병조제8 wk여제12 wk비교，승고차이역유통계학의의（P＜0．05）。<br> 결론：당뇨병조기출현료시망막신경절세포적조망，기궤제가능여세포내개리자농도승고유관。
AIM:To investigate the mechanism of retinal ganglion cell apoptosis in early stage of streptozotocin ( STZ )-induced diabetic rats. <br> METHODS: Sixty SD rats were randomly divided into two groups:control group ( CON) and diabetes mellitus group ( DM ) . Diabetic rat model was produced by intraperitoneal injection of 1% STZ in 30 adult male SD rats.At 4, 8, 12wk,the rats were killed and eyeballs were enucleated for the HE staining, TUNEL staining, transmission electron microscopy detection respectively, and laser confocal microscope detection was used to detect the calcium ion concentration. <br> RESULTS: At 8wk RGCs decreased gradually and appeared disordered arrangement and got worse at 12 wk in DM group.In DM group, mitochondrial swelling was detected at 4wk., and became more obvious, more in number at 8wk with reduction in some cells'volume and the number of organelles decreased.In DM group, few TUNEL positive RGCs were seen at 4wk, and became more and more at 8 and 12wk.The apoptosis index was significantly higher in DM group compared with CON group in different time points ( P<0.01).Intracellular calcium ion concentration of RGCs in DM group began to elevate obviously at 8 and 12wk in contrast with CON group in the same time point (P<0.01).In DM group, Intracellular calcium ion concentration of RGCs increased difference was statistically significant between 8 and 12wk (P<0.05). <br> CONCLUSION: The study suggested that RGCs apoptosis occurs in early stage of diabetes, the mechanism might be associated with increased intracellular calcium ion concentration.
