CAJ | 학술논문

目的：探讨荧光定量聚合酶链反应对造血干细胞移植患者人巨细胞病毒活动性感染的早期诊断价值。方法收集40例造血干细胞移植患者共416份血标本，应用实时荧光定量PCR方法动态监测血浆HCMV-DNA载量，并与60例健康体检者血浆HCMV-DNA载量对比分析。结果416份血标本中96份阳性，阳性率23.1%，病毒载量介于5.0×102~1.0×107拷贝/ml之间。结论实时荧光定量PCR方法检测HCMV-DNA适用于造血干细胞移植后巨细胞病毒活动性感染的早期诊断。
목적：탐토형광정량취합매련반응대조혈간세포이식환자인거세포병독활동성감염적조기진단개치。방법수집40례조혈간세포이식환자공416빈혈표본，응용실시형광정량PCR방법동태감측혈장HCMV-DNA재량，병여60례건강체검자혈장HCMV-DNA재량대비분석。결과416빈혈표본중96빈양성，양성솔23.1%，병독재량개우5.0×102~1.0×107고패/ml지간。결론실시형광정량PCR방법검측HCMV-DNA괄용우조혈간세포이식후거세포병독활동성감염적조기진단。
Objective To evaluate the usefulness of fluorescence quantitative PCR assay for early detection of HCMV activa-tion after hematopoietic stem cell transplantation. Methods Fluorescence quantitative PCR assay was used to monitor the HCMV-DNA load in 416 plasma samples from 40 bone marrow transplant recipients. 60 control plasma samples were collected for control samples. Results The levels of HCMV DNA copies of 96 positive samples in the 416 plasma samples ranged from 5.0×102 to1.0× 107copies/ml. Conclusion The quantitative detection of HCMV DNA by real-time PCR is a rapid method for monitoring HCMV infection and the early diagnosis in patients after hematopoietic stem cell transplantation.