CAJ | 학술논문

采用正交试验L16（43）设计，以dNTP、引物和Taq DNA聚合酶3种因素4个水平，并设置了8个模板DNA浓度梯度。扩增效果表明，红麻的SRAP-PCR最佳反应体系为2μL 10×Taq Reaction Buffer、20 ng模板DNA、 dNTP 220μmol/L、引物0.35μmol/L、 Taq DNA聚合酶0.5 U，总体积为20μL。同时对256对SRAP引物进行扩增，筛选出条带清晰、多态性较好的引物100对。该反应体系及100对多态性引物组合应用于今后红麻的遗传多样性、品种鉴定、亲缘关系、遗传图谱构建、 QTL定位等研究。
채용정교시험L16（43）설계，이dNTP、인물화Taq DNA취합매3충인소4개수평，병설치료8개모판DNA농도제도。확증효과표명，홍마적SRAP-PCR최가반응체계위2μL 10×Taq Reaction Buffer、20 ng모판DNA、 dNTP 220μmol/L、인물0.35μmol/L、 Taq DNA취합매0.5 U，총체적위20μL。동시대256대SRAP인물진행확증，사선출조대청석、다태성교호적인물100대。해반응체계급100대다태성인물조합응용우금후홍마적유전다양성、품충감정、친연관계、유전도보구건、 QTL정위등연구。
An orthogonal experiment L 16 ( 43 ) including three factors ( dNTP, primer and Taq DNA Polymerase ) and four levels was designed to optimize SRAP -PCR reaction system of Kenaf.Be-sides, we set up 8 concentration gradients of DNA template to test its influence on amplifying results.At last, we optimized the SRAP-PCR reaction system for Kenaf.It contained 2μL 10 ×Taq reaction buff-er, 20ng DNA template, 220μmol/L dNTP concentration, 0.35μmol/L primer concentration, 0.5U Taq DNA Polymerase , 20μL of the cumulative volume.In addition, 100 primer combinations were se-lected from 256 SRAP primer combinations due to their distinct Gershgorim bands and high polymor -phism.The optimal SRAP reaction system and 100 primer combinations would contribute to the basis for evaluating genetic diversity , identifing varieties , analyzing genetic relationship , building linkage map and QTL map for Hibiscus cannabinus L.