CAJ | 학술논문

目的：研制一种具备正常血管的生理结构和良好的生物相容性,而且具有血管力学特性的小口径组织工程血管基质材料,并按医用输血材料的力学及生物学国家标准评价。方法以去细胞生物衍生材料作为基质,按照国家标准对其进行力学和生物学评价。结果血管的轴向、径向拉伸应力分别为23.14 N和36.79 N,均大于标准规定的7.5 N；拉伸率分别为95.19%和80.24%,大于标准规定的20%。血管的缝合强度为13.71 N,不小于0.5 N,血管符合力学规定。生物学检测主要用血管或其浸提液检测血管的pH值在对照去离子水pH(7.52±1.5)范围内、溶血率1.3972%,小于5%、全血凝固时间比对照长50%、对细胞毒性为0级,皮内注射无刺激。结论血管基质材料具有良好的力学及生物学性能,它将在小口径血管替代治疗中起重要作用。
목적：연제일충구비정상혈관적생리결구화량호적생물상용성,이차구유혈관역학특성적소구경조직공정혈관기질재료,병안의용수혈재료적역학급생물학국가표준평개。방법이거세포생물연생재료작위기질,안조국가표준대기진행역학화생물학평개。결과혈관적축향、경향랍신응력분별위23.14 N화36.79 N,균대우표준규정적7.5 N；랍신솔분별위95.19%화80.24%,대우표준규정적20%。혈관적봉합강도위13.71 N,불소우0.5 N,혈관부합역학규정。생물학검측주요용혈관혹기침제액검측혈관적pH치재대조거리자수pH(7.52±1.5)범위내、용혈솔1.3972%,소우5%、전혈응고시간비대조장50%、대세포독성위0급,피내주사무자격。결론혈관기질재료구유량호적역학급생물학성능,타장재소구경혈관체대치료중기중요작용。
Objective To develop a small-diameter tissue-engineered blood vessels which possesses normal blood vessels physiological structure, good biocompatibility, and mechanical properties. And it was evaluated by mechanical and biological of national standard of medi-cal transfusion material. Methods The bio-derived material were regarded as the ground substance, and it was evaluated by mechanical and biological of national standard after composite modification. Results The axial and radial tensile stress of the blood vessel was 23. 14 N and 36. 79 N respectively, and it was greater than the standard 7. 5N. The tensile rate of the axial and radial was 95. 19% and 80. 24% respec-tively, which were higher than the standard value 20%. The suture strength of the blood vessel was 13. 71 N, which was conform to the me-chanical requirement. Mainly used blood vessels or its extracts to detect the pH of the blood vessels is in the scope of control deionized water pH (7. 5 ± 1. 5);the hemolysis rate was 1. 3972% which was less than 5%;the whole blood coagulation time was 50% longer than the con-trol level, and there was no stimulation after intradermal injection. Conclusion With bio-derived material as the ground substance and com-positely modified, this kind od blood vessels is conform to the mechanical and biological of national standard, and it has the potential of clini-cal application which could play an important role in the replacement therapy of small-diameter vascular xenografts.