CAJ | 학술논문

目的：探讨LATS1-YAP信号通路对人皮肤成纤维细胞活力及细胞外基质合成的调控作用。方法实验分为未干预组、LATS1 siRNA干预组和YAP siRNA干预组。构建LATS1 siRNA和YAP siRNA,LATS1 siRNA干预组利用LATS1 siRNA转染人皮肤成纤维细胞株HS27,YAP siRNA干预组利用YAP siRNA转染HS27。转染后48 h利用Western-blot观察LATS1、YAP和col-lagenⅠ的表达情况,应用MTT检测HS27细胞株的细胞活力情况。结果 LATS1 siRNA转染48 h后LATS1蛋白表达显著降低, YAP蛋白和collagenⅠ蛋白表达升高,细胞活力显著增高；YAP siRNA转染48 h后LATS1蛋白表达无变化,YAP蛋白和collagenⅠ蛋白表达降低,细胞活力显著降低。结论 LATS1-YAP信号通路可调控人皮肤成纤维细胞活力和细胞外基质的合成,可能成为皮肤创伤后修复以及阻止创伤后瘢痕形成提供潜在治疗靶点。
목적：탐토LATS1-YAP신호통로대인피부성섬유세포활력급세포외기질합성적조공작용。방법실험분위미간예조、LATS1 siRNA간예조화YAP siRNA간예조。구건LATS1 siRNA화YAP siRNA,LATS1 siRNA간예조이용LATS1 siRNA전염인피부성섬유세포주HS27,YAP siRNA간예조이용YAP siRNA전염HS27。전염후48 h이용Western-blot관찰LATS1、YAP화col-lagenⅠ적표체정황,응용MTT검측HS27세포주적세포활력정황。결과 LATS1 siRNA전염48 h후LATS1단백표체현저강저, YAP단백화collagenⅠ단백표체승고,세포활력현저증고；YAP siRNA전염48 h후LATS1단백표체무변화,YAP단백화collagenⅠ단백표체강저,세포활력현저강저。결론 LATS1-YAP신호통로가조공인피부성섬유세포활력화세포외기질적합성,가능성위피부창상후수복이급조지창상후반흔형성제공잠재치료파점。
Objective To study the proliferation of human skin fibroblast and synthesis of extracellular matrix which were regulated by LATS1-YAP pathway. Methods They were divided into three groups:control groups, LATS1 siRNA intervention group and YAP siRNA treatment group. Using LATS1 siRNA transferred human skin fibroblasts cell lines HS27 in LATS1 siRNA intervention group, and using YAP siRNA transferred HS27 in YAP siRNA treatment group. Expression of LATS1,YAP and collageⅠwere detected by western-blot 48 h later, and the activity of HS27 cells was determined by MTT. Results Compared with control group,expression of LATS1 protein decreased while expression of YAP protein and collagenⅠprotein increased 48 h after LATS1 siRNA transfection. Expression of LATS1 protein remains un-changed and expression of YAP protein and collagenⅠprotein decreased 48 h after YAP siRNA transfection. Conclusion LATS1-YAP pathway could regulate proliferation of human skin fibroblast and synthesis of extracellular matrix. It provides a potential therapeutic targets for skin wound repair and cicatrization.