局解手术学杂志
跼解手術學雜誌
국해수술학잡지
JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY
2014年
1期
1-4
,共4页
赵喆%姚远%杜长国%邓其跃
趙喆%姚遠%杜長國%鄧其躍
조철%요원%두장국%산기약
巨噬细胞迁移抑制因子%脊髓损伤%Ras同族基因家族成员A
巨噬細胞遷移抑製因子%脊髓損傷%Ras同族基因傢族成員A
거서세포천이억제인자%척수손상%Ras동족기인가족성원A
macrophage migration inhibitory factor%spinal cord injury%Ras homolog gene family member A
目的：探讨巨噬细胞迁移抑制因子(MIF)在小鼠脊髓损伤后的修复作用。方法选择C57BL/6小鼠制作重物砸伤的脊髓损伤模型,分为手术组和假手术对照组,损伤节段为T9~T10；用免疫组织化学法显示MIF在损伤急性期(术后72 h)的表达改变。用RT-PCR显示MIF与RhoA的mRNA水平改变；用免疫荧光双标显示小胶质细胞表达MIF和RhoA的情况。结果重物坠落致脊髓损伤后72 h,MIF在损伤段脊髓中蛋白表达量增加；同时MIF的mRNA水平升高,且伴随RhoA的mRNA水平升高,手术组和假手术组之间的差异有统计学意义(P<0.05)。结论重物坠落致脊髓损伤后的急性期,损伤局部活化的小胶质细胞中MIF表达增加；少突胶质和星形胶质样细胞中有MIF累积,神经元中MIF的水平变化不显著。同时,MIF的mRNA水平也出现上调,与RhoA的mRNA上调在时空上有一致性,提示MIF可能调节损伤局部微环境参与轴突再生抑制。
目的：探討巨噬細胞遷移抑製因子(MIF)在小鼠脊髓損傷後的脩複作用。方法選擇C57BL/6小鼠製作重物砸傷的脊髓損傷模型,分為手術組和假手術對照組,損傷節段為T9~T10；用免疫組織化學法顯示MIF在損傷急性期(術後72 h)的錶達改變。用RT-PCR顯示MIF與RhoA的mRNA水平改變；用免疫熒光雙標顯示小膠質細胞錶達MIF和RhoA的情況。結果重物墜落緻脊髓損傷後72 h,MIF在損傷段脊髓中蛋白錶達量增加；同時MIF的mRNA水平升高,且伴隨RhoA的mRNA水平升高,手術組和假手術組之間的差異有統計學意義(P<0.05)。結論重物墜落緻脊髓損傷後的急性期,損傷跼部活化的小膠質細胞中MIF錶達增加；少突膠質和星形膠質樣細胞中有MIF纍積,神經元中MIF的水平變化不顯著。同時,MIF的mRNA水平也齣現上調,與RhoA的mRNA上調在時空上有一緻性,提示MIF可能調節損傷跼部微環境參與軸突再生抑製。
목적：탐토거서세포천이억제인자(MIF)재소서척수손상후적수복작용。방법선택C57BL/6소서제작중물잡상적척수손상모형,분위수술조화가수술대조조,손상절단위T9~T10；용면역조직화학법현시MIF재손상급성기(술후72 h)적표체개변。용RT-PCR현시MIF여RhoA적mRNA수평개변；용면역형광쌍표현시소효질세포표체MIF화RhoA적정황。결과중물추락치척수손상후72 h,MIF재손상단척수중단백표체량증가；동시MIF적mRNA수평승고,차반수RhoA적mRNA수평승고,수술조화가수술조지간적차이유통계학의의(P<0.05)。결론중물추락치척수손상후적급성기,손상국부활화적소효질세포중MIF표체증가；소돌효질화성형효질양세포중유MIF루적,신경원중MIF적수평변화불현저。동시,MIF적mRNA수평야출현상조,여RhoA적mRNA상조재시공상유일치성,제시MIF가능조절손상국부미배경삼여축돌재생억제。
Objective To study the effects of macrophage migration inhibitory factor (MIF) on spinal cord injury. Methods C57BL/6 mice with injury at T9 ~T10 were taken as spinal cord injury ( SCI) model, and they were divided into the operated group and the sham oper-ated group. Immunohistochemistry was used to detect expression changes of MIF during 72 hours after operation. Reverse transcriptase-poly-merase chain reaction ( RT-PCR) was used to test changes of mRNA level. And double staining immunofluorescence techniques was used to show expressions of MIF and RhoA. Results Expression of MIF at acute stage (72 hours after injury) increased significantly. Results of RT-PCR showed that mRNA levels of MIF and RhoA both increased. There were statistical differences between the two groups (P<0. 05). Con-clusion In acute stage after SCI, MIF increased in the activated microglia. MIF accumulated in oligodendrocyte-like and astrocyte-like cells in injured spinal cord, which might contribute to inhibitory environment for regeneration. Moreover, mRNA level of MIF raised with the in-crease of RhoA mRNA level, which indicated the potential inhibition of MIF to axonal regeneration in CNS.
