皖南医学院学报
皖南醫學院學報
환남의학원학보
ACTA ACADEMIAE MEDICINAE WANNAN
2014年
1期
23-26
,共4页
滁菊药渣%多糖%纤维素酶%提取%抗氧化性
滁菊藥渣%多糖%纖維素酶%提取%抗氧化性
저국약사%다당%섬유소매%제취%항양화성
Dendranthema morifolium residue%polysaccharides%cellulase%extraction%antioxidant activities
目的：从滁菊醇提药渣中用纤维素酶法提取多糖，研究滁菊多糖抗氧化性。方法：分别考察酶解pH值、酶解温度、酶用量、酶解时间4个因素对滁菊多糖得率的影响。结果：通过正交试验得出滁菊多糖的最佳提取工艺为：酶解pH 5．5、温度60℃、酶用量20 U/g、酶解时间100 min，多糖得率为13．89％。结论：对DPPH自由基和超氧阴离子自由基的清除率表明滁菊多糖具有较好的抗氧化活性。
目的：從滁菊醇提藥渣中用纖維素酶法提取多糖，研究滁菊多糖抗氧化性。方法：分彆攷察酶解pH值、酶解溫度、酶用量、酶解時間4箇因素對滁菊多糖得率的影響。結果：通過正交試驗得齣滁菊多糖的最佳提取工藝為：酶解pH 5．5、溫度60℃、酶用量20 U/g、酶解時間100 min，多糖得率為13．89％。結論：對DPPH自由基和超氧陰離子自由基的清除率錶明滁菊多糖具有較好的抗氧化活性。
목적：종저국순제약사중용섬유소매법제취다당，연구저국다당항양화성。방법：분별고찰매해pH치、매해온도、매용량、매해시간4개인소대저국다당득솔적영향。결과：통과정교시험득출저국다당적최가제취공예위：매해pH 5．5、온도60℃、매용량20 U/g、매해시간100 min，다당득솔위13．89％。결론：대DPPH자유기화초양음리자자유기적청제솔표명저국다당구유교호적항양화활성。
Objective:To tentatively verify the process for further extracting the polysaccharides from flower residue of Dendranthema morifolium Tzvel. (DMP,treated previously with ethanol) with cellulase degradation technique and assay the antioxidant activities of DMP.Methods:The influential factors affecting the recovery rate in extracting polysaccharides were determined regarding the pH value,temperature at enzymolysis,enzyme dosage and extraction time.Results:By orthogonal test analysis,the results showed that the optimal conditions were involved in pH value by 5.5,temperature at 60 ℃,enzyme dosage in 20 U/g and extraction time for 100 min in a typical full extracting process,which led to recovery rate of 13.89%.Conclusion:The experimental findings show that DMP may facilitate stronger antioxidant activities based on measurement of the scavenging effect on DPPH and superoxide anion free radical.
