CAJ | 학술논문

目的：检测多发性骨髓瘤（Multiple myeloma，MM）患者及正常人外周血和骨髓Treg细胞水平，评价MM患者骨髓微环境的免疫状态，以及免疫状态与疾病水平的相关性。方法采用流式细胞术检测45例MM患者外周血以及骨髓中Treg细胞水平，15例正常人外周血及骨髓中Treg细胞水平。以CD4＋CD25high＋细胞占CD4＋细胞的百分比代表Treg细胞水平。结果 MM患者外周血Treg细胞高于正常人外周血，MM患者骨髓中Treg细胞低于正常人骨髓，MM患者骨髓微环境中Treg细胞降低水平与浆细胞比例无关，而与疾病的预后相关，与β2-MG水平及ISS相关。结论 MM骨髓微环境中肿瘤诱导的免疫系统的改变是MM发病的原因之一，MM患者骨髓中Treg细胞降低比例与预后相关，而与肿瘤负担无关。
목적：검측다발성골수류（Multiple myeloma，MM）환자급정상인외주혈화골수Treg세포수평，평개MM환자골수미배경적면역상태，이급면역상태여질병수평적상관성。방법채용류식세포술검측45례MM환자외주혈이급골수중Treg세포수평，15례정상인외주혈급골수중Treg세포수평。이CD4＋CD25high＋세포점CD4＋세포적백분비대표Treg세포수평。결과 MM환자외주혈Treg세포고우정상인외주혈，MM환자골수중Treg세포저우정상인골수，MM환자골수미배경중Treg세포강저수평여장세포비례무관，이여질병적예후상관，여β2-MG수평급ISS상관。결론 MM골수미배경중종류유도적면역계통적개변시MM발병적원인지일，MM환자골수중Treg세포강저비례여예후상관，이여종류부담무관。
Objective To evaluate immune function of patients with multiple myeloma ( MM) by detect the changes of the Treg cells in bone marrow of patients with MM .Methods Treg cells in peripheral blood and bone marrow of 45 patients with MM and 15 healthy controls were measured by flow cytometry .Results The per-centage of Treg cells in peripheral blood of MM was higher than that in healthy person ;The percentage of Treg cells in MM bone marrow was lower than that in healthy person ,and the extent was positively correlated with the level of β2-MG and ISS(P<0.05).Conclusion MM Patients display an abnormal level of CD 4 +CD25 +Treg cells,which may be an important mechanism of MM immune deficiency .