生物技术通讯
生物技術通訊
생물기술통신
LETTERS IN BIOTECHNOLOGY
2014年
1期
68-70
,共3页
巩伟丽%刘朝山%谭博%韩秋颖%陈亮
鞏偉麗%劉朝山%譚博%韓鞦穎%陳亮
공위려%류조산%담박%한추영%진량
视网膜母细胞瘤结合蛋白6%NF-κB%转录活性
視網膜母細胞瘤結閤蛋白6%NF-κB%轉錄活性
시망막모세포류결합단백6%NF-κB%전록활성
retinoblastoma binding protein 6%NF-κB%transcriptional activity
目的：研究视网膜母细胞瘤结合蛋白6（RbBP6）对TNF-α激活的NF-κB转录活性的影响。方法：构建RbBP6的真核表达载体并在293T细胞中过表达，或利用RNA干扰技术敲低RbBP6的表达，采用NF-κB双萤光素酶报告系统检测RbBP6对TNF-α激活的NF-κB转录活性的影响。结果：过表达RbBP6对NF-κB转录活性无明显影响，而瞬时干扰RbBP6能够明显抑制NF-κB的转录活性。结论：实验结果提示RbBP6在NF-κB信号通路中可能发挥重要调控作用。
目的：研究視網膜母細胞瘤結閤蛋白6（RbBP6）對TNF-α激活的NF-κB轉錄活性的影響。方法：構建RbBP6的真覈錶達載體併在293T細胞中過錶達，或利用RNA榦擾技術敲低RbBP6的錶達，採用NF-κB雙螢光素酶報告繫統檢測RbBP6對TNF-α激活的NF-κB轉錄活性的影響。結果：過錶達RbBP6對NF-κB轉錄活性無明顯影響，而瞬時榦擾RbBP6能夠明顯抑製NF-κB的轉錄活性。結論：實驗結果提示RbBP6在NF-κB信號通路中可能髮揮重要調控作用。
목적：연구시망막모세포류결합단백6（RbBP6）대TNF-α격활적NF-κB전록활성적영향。방법：구건RbBP6적진핵표체재체병재293T세포중과표체，혹이용RNA간우기술고저RbBP6적표체，채용NF-κB쌍형광소매보고계통검측RbBP6대TNF-α격활적NF-κB전록활성적영향。결과：과표체RbBP6대NF-κB전록활성무명현영향，이순시간우RbBP6능구명현억제NF-κB적전록활성。결론：실험결과제시RbBP6재NF-κB신호통로중가능발휘중요조공작용。
Objective: In order to research the effect of retinoblastoma binding protein 6(RbBP6) on the tran-scriptional activity of NF-κB under the stimuli of TNF-α. Methods: We constructed eukaryotic expression vector of RbBP6 and overexpressed it in 293T cells, also we knockdowned the expression of RbBP6 by RNAi, and dual-luciferase reporter assay system was used to evaluate the effect of RbBP6 on transcriptional activity of NF-κB un-der the stimuli of TNF-α. Results: Overexpression of RbBP6 had little effect on the activation of NF-κB, while knockdown of it could inhibit the transcriptional activity of NF-κB. Conclusion: The results indicated that RbBP6 may play important roles in the regulation of the NF-κB signaling pathway.
