CAJ | 학술논문

目的：从新疆石河子盐碱地菊芋生长根际土壤中分离筛选高产菊粉酶活力菌株。方法：通过稀释平板涂布法分离微生物；利用60Co诱变选育，96孔板筛选突变菌株；采用3，5-二硝基水杨酸比色法测定菊粉酶酶活。结果：分离到12株具有菊粉酶活力的菌株，复筛得到1株高产菊粉酶活力菌株，将其命名为G-60；以此菌株为出发菌株进行60Co诱变，利用96孔板对诱变菌株进行筛选，经摇瓶发酵酶活测定，得到1株高产菊粉酶酶活的突变株，酶活达46.62 U/mL，是未诱变菌株酶活的2.72倍。结论：经诱变得到1株高产菊粉酶活力的突变菌株。
목적：종신강석하자염감지국우생장근제토양중분리사선고산국분매활력균주。방법：통과희석평판도포법분리미생물；이용60Co유변선육，96공판사선돌변균주；채용3，5-이초기수양산비색법측정국분매매활。결과：분리도12주구유국분매활력적균주，복사득도1주고산국분매활력균주，장기명명위G-60；이차균주위출발균주진행60Co유변，이용96공판대유변균주진행사선，경요병발효매활측정，득도1주고산국분매매활적돌변주，매활체46.62 U/mL，시미유변균주매활적2.72배。결론：경유변득도1주고산국분매활력적돌변균주。
Objective: To screen strains with high inulinase productivity, which were collected from the root soil of Jerusalem artichoke areas in Shihezi City, Xinjiang, China. Methods: The microorganisms were isolated by the method of diluting and plating. 60Co mutagenic treatment and 96 cell culture plate were used to screen the mutant strains. The inulinase activity was determined by using 3, 5-dinitro salicylic acid colorimetry. Results: 12 strains with inulinase productivity were screened, and 1 strain with high inulinase activity was named G-60. The G-60 was as starting strain to conduct 60Co mutagenic treatment and 96 cell culture plate to screen the mutant strains, and 1 mutation stain with high inulinase activity was gained through shaking flask fermentation, which the inulin-ase activity reached 46.62 U/mL, being 2.72 times that of the starting strains. Conclusion: After mutagenesis we get a high inulinase mutant strain.