食品与发酵科技
食品與髮酵科技
식품여발효과기
SICHUAN FOOD AND FERMENTATION
2014年
1期
48-50,74
,共4页
双歧杆菌%乳酸杆菌%混合发酵
雙歧桿菌%乳痠桿菌%混閤髮酵
쌍기간균%유산간균%혼합발효
Bifidobacteria%Lactobacteria%mixed fermentation
本文通过正交试验,首先优化双歧杆菌和乳酸杆菌混合发酵工艺,确定得出其最适发酵条件为:初始pH值为7.2,培养温度为35℃,最佳培养基配方为酵母膏0.9%,胰蛋白胨0.6%,大豆蛋白胨0.3%,葡萄糖2.5%,双歧因子(低聚果糖)0.3%,生长因子5%。在此基础上,在100L发酵罐规模,进行了乳酸菌和双歧杆菌通过上述条件混合发酵中实验试,实验结果显示,发酵液中双歧杆菌、乳酸杆菌发酵液的活菌数均达到每mL在10亿/mL个以上,可以满足产业化需要。
本文通過正交試驗,首先優化雙歧桿菌和乳痠桿菌混閤髮酵工藝,確定得齣其最適髮酵條件為:初始pH值為7.2,培養溫度為35℃,最佳培養基配方為酵母膏0.9%,胰蛋白胨0.6%,大豆蛋白胨0.3%,葡萄糖2.5%,雙歧因子(低聚果糖)0.3%,生長因子5%。在此基礎上,在100L髮酵罐規模,進行瞭乳痠菌和雙歧桿菌通過上述條件混閤髮酵中實驗試,實驗結果顯示,髮酵液中雙歧桿菌、乳痠桿菌髮酵液的活菌數均達到每mL在10億/mL箇以上,可以滿足產業化需要。
본문통과정교시험,수선우화쌍기간균화유산간균혼합발효공예,학정득출기최괄발효조건위:초시pH치위7.2,배양온도위35℃,최가배양기배방위효모고0.9%,이단백동0.6%,대두단백동0.3%,포도당2.5%,쌍기인자(저취과당)0.3%,생장인자5%。재차기출상,재100L발효관규모,진행료유산균화쌍기간균통과상술조건혼합발효중실험시,실험결과현시,발효액중쌍기간균、유산간균발효액적활균수균체도매mL재10억/mL개이상,가이만족산업화수요。
The optimum formula fermented by Bifidobacteria and Lactobacteria together was optimized by orthogonal experiment in this paper. The optimal fermentation condition were obtained, which involved 35℃ fermentation temperature, pH 7.2, the optimum composition for the fermentation medium was: yeast extract 0.9%, tryptone 0.6%, soy peptone 0.3%, glucose 2.5%, bifidus factor (fructo-oligose) 0.3%, growth factor 5%. Cultivated in 10L and 100L fermentor under the optimal fermentation condition the viable counts of Bifidobacterium and Lactobacillus reached up to 109CFU/mL, which can absolutely meet the needs of the industrialization.