食品与发酵科技
食品與髮酵科技
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SICHUAN FOOD AND FERMENTATION
2014年
1期
19-24
,共6页
羽毛降解%角蛋白酶%响应面设计
羽毛降解%角蛋白酶%響應麵設計
우모강해%각단백매%향응면설계
feather degradation%Keratinase%response surface methodology
为提高Bacillus pumilus ZW-36产角蛋白降解酶能力，考察了初始pH、羽毛浓度、无机盐、外加碳氮源等因数对其产角蛋白酶的影响。采用单因素试验和响应面分析法的Box-Behnken进行试验设计，建立了FeSO4、MgSO4和K2HPO4的二次回归模型。得到产角蛋白酶的最佳条件为：培养基初始pH 6.5、羽毛0.5％、NaCl 0.5％、FeSO40.036％、MgSO40.025％、K2HPO40.073％、KH2PO40.035％，玉米粉0.5％。在此条件下发酵36h角蛋白酶活达到57.14U/mL，达到模型预测值的96.7％，较优化前（38.27U/mL）酶活提高了49.3％。
為提高Bacillus pumilus ZW-36產角蛋白降解酶能力，攷察瞭初始pH、羽毛濃度、無機鹽、外加碳氮源等因數對其產角蛋白酶的影響。採用單因素試驗和響應麵分析法的Box-Behnken進行試驗設計，建立瞭FeSO4、MgSO4和K2HPO4的二次迴歸模型。得到產角蛋白酶的最佳條件為：培養基初始pH 6.5、羽毛0.5％、NaCl 0.5％、FeSO40.036％、MgSO40.025％、K2HPO40.073％、KH2PO40.035％，玉米粉0.5％。在此條件下髮酵36h角蛋白酶活達到57.14U/mL，達到模型預測值的96.7％，較優化前（38.27U/mL）酶活提高瞭49.3％。
위제고Bacillus pumilus ZW-36산각단백강해매능력，고찰료초시pH、우모농도、무궤염、외가탄담원등인수대기산각단백매적영향。채용단인소시험화향응면분석법적Box-Behnken진행시험설계，건립료FeSO4、MgSO4화K2HPO4적이차회귀모형。득도산각단백매적최가조건위：배양기초시pH 6.5、우모0.5％、NaCl 0.5％、FeSO40.036％、MgSO40.025％、K2HPO40.073％、KH2PO40.035％，옥미분0.5％。재차조건하발효36h각단백매활체도57.14U/mL，체도모형예측치적96.7％，교우화전（38.27U/mL）매활제고료49.3％。
In order to enhance the Keratinase activity of Bacillus pumilus strain ZW-36, the influence of initial pH, concentration of feathers, inorganic salt, external carbon source, external nitrogen source on Keratinase production were studied. Designed with single factor experiment and the Box-Behnken in the response surface methodology, a quadratic regression model of ferrous sulfate, magnesium sulfate and dipotassium phosphate was established. The optimization conditions of producing Keratinase were initial pH at 6.5, 0.5%of chicken feather, 0.5% of sodium chloride, 0.036% of ferrous sulfate, 0.025% of magnesium sulfate, 0.073%of dipotassium phosphate, 0.035% of potassium dihydrogen phosphate and 0.5%of corn flour. Under this conditions, the Keratinase activity in fermentation broth of shask on the 36 hours was 57.14U/mL, which was higher 49.3% than before.
