CAJ | 학술논문

目的：探讨利用血浆中游离DNA进行短串联重复序列（short tandem repeat，STR）分型检测，解决法医学个体识别和亲权鉴定问题的可行性。方法采集36例无关健康个体EDTA-Na2抗凝血样，分离血浆，采用经典酚-氯仿法分别处理血浆和血细胞，对提取的DNA进行15个STR基因座常规PCR扩增和荧光标记复合扩增，采用聚丙烯酰胺凝胶电泳和毛细管电泳检测2种STR分型方法进行检测。结果常规PCR扩增银染检测和荧光标记复合扩增毛细管电泳检测两种STR分型方法的结果表明，同一个体的血浆游离DNA和血细胞DNA STR分型一致，且分型效果接近。结论血浆游离DNA可作为一种有效的生物学样本进行STR分型检测，应用于法医个体识别和亲权鉴定。
목적：탐토이용혈장중유리DNA진행단천련중복서렬（short tandem repeat，STR）분형검측，해결법의학개체식별화친권감정문제적가행성。방법채집36례무관건강개체EDTA-Na2항응혈양，분리혈장，채용경전분-록방법분별처리혈장화혈세포，대제취적DNA진행15개STR기인좌상규PCR확증화형광표기복합확증，채용취병희선알응효전영화모세관전영검측2충STR분형방법진행검측。결과상규PCR확증은염검측화형광표기복합확증모세관전영검측량충STR분형방법적결과표명，동일개체적혈장유리DNA화혈세포DNA STR분형일치，차분형효과접근。결론혈장유리DNA가작위일충유효적생물학양본진행STR분형검측，응용우법의개체식별화친권감정。
Objective The purpose of this study was to investigate the feasibility of short tandem repeat(STR) genotyping of cell free DNA in plasma for individual identification and paternity testing. Methods EDTA-Na2 DNA anti-coagulant blood samples were collected from 36 unrelated healthy volunteers,and both DNA in leukocytes and cell free DNA in plasma were extracted respectively using phenol-chloroform method. Target DNA in blood cells and plasma were amplified using regular STR typing and fluorescent multiplex STR assay separately,accordingly,the PCR products were analyzed by polyacrylamide gel electrophoresis and capillary electrophoresis. Results Using either normal PCR-STR or fluorescent multiplex STR assay,the consistent STR genotyping results were detected with similar efficiency for cell DNA and plasma DNA samples from the same individual. Conclusion Cell free DNA in plasma samples can be used as useful biological samples for STR genotyping,which can be applied to individual identification and paternity testing in forensic practice.