CAJ | 학술논문

[目的]观察由内皮素1激活的内皮素A受体对家兔动脉粥样硬化斑块C反应蛋白表达的影响。[方法]32只雄性新西兰大白兔完全随机设计分成基础组、模型组、BQ-123组以及辛伐他汀组，每组8只。饲喂10周后，采耳动脉血检测血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）以及低密度脂蛋白胆固醇（LDL-C）水平；采用免疫组织化学SP法检测主动脉标本中组织内皮素1（ET-1）、巨噬细胞及C反应蛋白（CRP）表达。[结果]模型组、BQ-123组及辛伐他汀组血清 TC、TG、LDL-C均高于基础组（ P均＜0．01）。BQ-123组及辛伐他汀组血清TC、TG、LDL-C均低于模型组（ P均＜0．01）。模型组、BQ-123组及辛伐他汀组血清HDL-C低于基础组（ P均＜0．01）。BQ-123组及辛伐他汀组血清HDL-C高于模型组（ P均＜0．01）。BQ-123组及辛伐他汀组家兔动脉粥样硬化斑块ET-1、巨噬细胞源泡沫细胞、CRP阳性表达均较模型组明显降低，BQ-123组家兔动脉粥样硬化斑块ET-1、巨噬细胞源泡沫细胞、CRP阳性表达又较辛伐他汀组进一步降低。[结论]ET-1激活的ET受体A（ETRA）可以导致家兔动脉粥样硬化斑块CRP表达的上调；选择性ETRA拮抗剂（ETRAA）可以预防家兔动脉粥样硬化进程的发展。
[목적]관찰유내피소1격활적내피소A수체대가토동맥죽양경화반괴C반응단백표체적영향。[방법]32지웅성신서란대백토완전수궤설계분성기출조、모형조、BQ-123조이급신벌타정조，매조8지。사위10주후，채이동맥혈검측혈청총담고순（TC）、삼선감유（TG）、고밀도지단백담고순（HDL-C）이급저밀도지단백담고순（LDL-C）수평；채용면역조직화학SP법검측주동맥표본중조직내피소1（ET-1）、거서세포급C반응단백（CRP）표체。[결과]모형조、BQ-123조급신벌타정조혈청 TC、TG、LDL-C균고우기출조（ P균＜0．01）。BQ-123조급신벌타정조혈청TC、TG、LDL-C균저우모형조（ P균＜0．01）。모형조、BQ-123조급신벌타정조혈청HDL-C저우기출조（ P균＜0．01）。BQ-123조급신벌타정조혈청HDL-C고우모형조（ P균＜0．01）。BQ-123조급신벌타정조가토동맥죽양경화반괴ET-1、거서세포원포말세포、CRP양성표체균교모형조명현강저，BQ-123조가토동맥죽양경화반괴ET-1、거서세포원포말세포、CRP양성표체우교신벌타정조진일보강저。[결론]ET-1격활적ET수체A（ETRA）가이도치가토동맥죽양경화반괴CRP표체적상조；선택성ETRA길항제（ETRAA）가이예방가토동맥죽양경화진정적발전。
[Objective]To observe the effect of endothelin receptor A activated by endothelin-1(ET-1) on the expression of C-reactive protein(CRP) in rabbits with atherosclerotic plaque .[Methods] Thirty-two male New Zealand white rabbits were randomly divided into baseline group ,model group ,BQ-123 group and simv-astatin group with 8 rabbits in each group .After 10 weeks ,the peripheral blood was collected from ear artery to determine the levels of total cholesterol (TC) ,triglyceride(TG) ,high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C) in serum .SP immunohistochemical staining was used to detect the expression of ET-1 ,macrophage and CRP in aorta .[Results] Serum levels of TC ,TG and LDL-C in model group ,BQ-123 group and simvastatin group were significantly higher than those in baseline group(all P<0 .01) ,while serum levels of TC ,TG and LDL-C in BQ-123 group and simvastatin group were significant-ly lower than those in model group(all P<0 .01) .Serum levels of HDL-C in model group ,BQ-123 group and simvastatin group were significantly lower than those in baseline group (all P<0 .01) .Serum levels of HDL-C in BQ-123 group and simvastatin group were higher than those in model group (all P <0 .01) .Compared with model group ,the positive expression of ET-1 ,macrophage-derived foam cells and CRP in BQ-123 group and simvastatin group decreased obviously .Compared with simvastatin group ,the positive expression of ET-1 , macrophage-derived foam cells and CRP in BQ-123 group further decreased .[Conclusion] Endothelin receptor A activated by ET-1 can up regulate the expression of CRP protein in atherosclerotic plaque of rabbits .Selec-tive endothelin receptor A antagonist can prevent the development of atherosclerosis in rabbits .