CAJ | 학술논문

[目的]探讨地塞米松对α-萘异硫氰酸（ANIT ）诱导的胆汁淤积大鼠的治疗作用。[方法]将健康雄性Wistar大鼠32只随机分为A、B、C、D四组。A组，仅灌花生油。B组，仅灌ANIT液。C组，灌ANIT液的同时予地塞米松（DEM ）处理。D组，灌ANIT液后24 h予DEM处理，每组8只。48 h后收集胆汁，计1 h胆汁量（BC）。取门静脉血检测总胆红素（TBIL），直接胆红素（DBIL）碱性磷酸酶（ALP），丙氨酸转氨酶（ALT ）的浓度。肝脏标本活检。[结果]B组各项指标明显高于A组（ P ＜0．01）。C、D两组 TBIL、DBIL指标低于B组（P ＜0．01），D组与C组两组无明显区别（P ＞0．05），D组略高于C组。B组ALP高于C组（P＜0．05），低于D组（ P＜0．01）。D组高于C组（ P ＜0．01）。B组ALT低于C组（ P ＜0．01）。C、D两组没有明显差别（P＞0．05）。B、D两组没有明显差别（P ＞0．05）。B、C、D三组中，BC值C组多于B、D组（P＜0．01）。D组多于B组（ P ＜0．01）。[结论]地塞米松有减黄作用，提示临床对药物性肝损引起的急性黄疸病例可早期予激素制剂干预。鉴于激素干预可能增加肝细胞损伤，大剂量应用应该谨慎。
[목적]탐토지새미송대α-내이류청산（ANIT ）유도적담즙어적대서적치료작용。[방법]장건강웅성Wistar대서32지수궤분위A、B、C、D사조。A조，부관화생유。B조，부관ANIT액。C조，관ANIT액적동시여지새미송（DEM ）처리。D조，관ANIT액후24 h여DEM처리，매조8지。48 h후수집담즙，계1 h담즙량（BC）。취문정맥혈검측총담홍소（TBIL），직접담홍소（DBIL）감성린산매（ALP），병안산전안매（ALT ）적농도。간장표본활검。[결과]B조각항지표명현고우A조（ P ＜0．01）。C、D량조 TBIL、DBIL지표저우B조（P ＜0．01），D조여C조량조무명현구별（P ＞0．05），D조략고우C조。B조ALP고우C조（P＜0．05），저우D조（ P＜0．01）。D조고우C조（ P ＜0．01）。B조ALT저우C조（ P ＜0．01）。C、D량조몰유명현차별（P＞0．05）。B、D량조몰유명현차별（P ＞0．05）。B、C、D삼조중，BC치C조다우B、D조（P＜0．01）。D조다우B조（ P ＜0．01）。[결론]지새미송유감황작용，제시림상대약물성간손인기적급성황달병례가조기여격소제제간예。감우격소간예가능증가간세포손상，대제량응용응해근신。
[Objective]To explore the role of dexamethasone(DEM ) in the treatment of rats with cholesta-sis induced by α-isothiocyanate(ANIT) .[Methods]A total of 32 healthy adult male Wistar rats were randomly divided into group A ,B ,C and D with 8 rats in each group .Group A was treated with peanut oil only .Group B was infused with ANIT only .Group C was infused with ANIT and DEM concurrently .Group D was infused with DEM after infusion with ANIT for 24h .The 1h bile count(BC) was calculated .Portal venous blood was drawn to detect the levels of total bilirubin (TBIL) ,direct bilirubin(DBIL) ,alkaline phosphatase(ALP) and alanine transaminase(ALT) .Biopsy of liver samples was performed .[Results]The indicators of group B were obviously higher than those of group A( P= <0 .01) .The TBIL and DBIL of group C and group D were low-er than those of group B( P<0 .01) ,but there was no significant difference between group D and group C ( P>0 .05) ,and those in group B were slightly higher than those in group C .The ALP of group B was higher than that of group C( P<0 .05) ,but lower than that of group D( P<0 .01) ,and that of group D was higher than that of group C( P<0 .01) .The ALT of group B was lower than that of group C( P<0 .01) ,but there was no significant difference between group C and group D ( P>0 .05) ,and there was no significant difference between group B and group D( P>0 .05) .The BC value of group C was higher than that of group B and D ( P<0 .01) ,and that of group D was higher than that of group B ( P <0 .01) .[Conclusion] DEM can decrease jaundice .It suggests that early intervention with glucocorticoid agents should be taken for clinical cases of a-cute jaundice induced by drug-induced liver injury .Hormonal intervention may increase liver cell damage ,so high dose usage should be cautious .