CAJ | 학술논문

目的：探讨羊水细胞培养对孕中期孕妇进行产前诊断的可行性及必要性，防止染色体病患儿的出生。方法对293例有产前诊断指征的孕妇进行羊膜腔穿刺，采取羊水细胞培养，制备中期染色体，分析胎儿核型，进行产前诊断。结果发现染色体异常14例，异常率4．78％，其中数目异常9例，结构异常5例，不同产前诊断指征分组中的异常率不同，以夫妇一方为染色体异常组异常率最高。结论妊娠中期对有产前诊断指征的孕妇进行羊水细胞培养染色体核型分析是产前诊断的重要手段，而血清学筛查，染色体检查和超声检查三者相互结合对于预防出生缺陷意义重大。
목적：탐토양수세포배양대잉중기잉부진행산전진단적가행성급필요성，방지염색체병환인적출생。방법대293례유산전진단지정적잉부진행양막강천자，채취양수세포배양，제비중기염색체，분석태인핵형，진행산전진단。결과발현염색체이상14례，이상솔4．78％，기중수목이상9례，결구이상5례，불동산전진단지정분조중적이상솔불동，이부부일방위염색체이상조이상솔최고。결론임신중기대유산전진단지정적잉부진행양수세포배양염색체핵형분석시산전진단적중요수단，이혈청학사사，염색체검사화초성검사삼자상호결합대우예방출생결함의의중대。
Objective To investigate the feasibility and necessity of amniotic fluid cells culture in the prenatal diagnosis for chromosomal diseases in order to prevent the birth of fetus with chromosomal diseases .Methods The 293 specimens of amniotic cell obtained by amniocentesis from 293 pregnant women with prenatal diagnosis indications were cultured and analyzed .Results Among the 293 specimens of amniotic cell ,14 cases of chromosome abnormality were found , with the chromosome abnormality rate being 4.78%,including 9 cases of chromosome number abnormality and 5 cases of chromosomal structure abnormality .The chromosome abnormality rates were different in different groups with different prenatal diagnosis indications ,in which the chromosome abnormality rates were the highest in the group with one party of the couple with chromosome abnormality .Conclusion The amniotic cell culture and chromosome karyotype analysis are the effective means in prenatal diagnosis for chromosomal diseases in the pregnant women with prenatal diagnosis indications at mid trimester of pregnancy, moreover, the combined detection of serological screening , chromosome examination and ultrasonic inspection plays an important role in the prevention of child birth defects .