CAJ | 학술논문

扩张床吸附是一种新型的集固液分离、浓缩、纯化于一体的层析技术，其核心为经过特殊设计的吸附介质。比较了Streamline SP、 Streamline SP XL和 Fastline SP三种常用扩张床介质的理化性质和床层特性，并以乳铁蛋白为模型蛋白，从静态吸附、吸附动力学和动态吸附三方面比较了不同介质的吸附特性。结果表明，介质颗粒小，吸附平衡快；介质密度大，床层稳定性高，适用流速大，过程处理能力高，研究的结果为扩张床吸附介质的理性设计提供指导。
확장상흡부시일충신형적집고액분리、농축、순화우일체적층석기술，기핵심위경과특수설계적흡부개질。비교료Streamline SP、 Streamline SP XL화 Fastline SP삼충상용확장상개질적이화성질화상층특성，병이유철단백위모형단백，종정태흡부、흡부동역학화동태흡부삼방면비교료불동개질적흡부특성。결과표명，개질과립소，흡부평형쾌；개질밀도대，상층은정성고，괄용류속대，과정처리능력고，연구적결과위확장상흡부개질적이성설계제공지도。
Expanded bed adsorption (EBA) is a challenge bioseparation approach, which integrates the solid-liquid separation, concentration and primary purification into a unit operation. The key to the EBA is the specially-designed adsorbents. In the present work, the physical and chemical properties of three widely-used EBA adsorbents named Streamline SP, Streamline SP XL and Fastline SP were compared. The expansion characteristic and liquid mixing property of the beds formed by using above three adsorbents separately were compared too. With lactoferrin as the model protein, the adsorption properties including adsorption isotherms, adsorption kinetics and breakthrough curves of the three adsorbents were investigated. The results indicate that the adsorbent with small size can come to adsorption equilibrium faster and the adsorbent with high density could improve the bed stability and allow higher flow rate, which enhances the treating ability of the bed. This work would provide useful information for the design of new EBA adsorbents.