分析仪器
分析儀器
분석의기
ANALYTICAL INSTRUMENTATION
2014年
1期
17-22
,共6页
马玉婷%严心涛%陈忠祥%王策%武晓东
馬玉婷%嚴心濤%陳忠祥%王策%武曉東
마옥정%엄심도%진충상%왕책%무효동
流式细胞术%层流%聚焦%流动室
流式細胞術%層流%聚焦%流動室
류식세포술%층류%취초%류동실
flow cytometry%laminar%focus%flow cell
使用FL U EN T对流式细胞仪液流系统中的核心部件流动室进行了流体仿真分析。探讨了样品流速、鞘液流速、进样针位置对聚焦效果的影响,分析了鞘液不对称进样的实施可能性,以及对鞘液脉动性的补偿措施。搭建了流式平台进行荧光微球的CV值测定,其变化趋势与仿真结果较为吻合。仿真结果为流式细胞仪液流系统的设计提供了参考依据。
使用FL U EN T對流式細胞儀液流繫統中的覈心部件流動室進行瞭流體倣真分析。探討瞭樣品流速、鞘液流速、進樣針位置對聚焦效果的影響,分析瞭鞘液不對稱進樣的實施可能性,以及對鞘液脈動性的補償措施。搭建瞭流式平檯進行熒光微毬的CV值測定,其變化趨勢與倣真結果較為吻閤。倣真結果為流式細胞儀液流繫統的設計提供瞭參攷依據。
사용FL U EN T대류식세포의액류계통중적핵심부건류동실진행료류체방진분석。탐토료양품류속、초액류속、진양침위치대취초효과적영향,분석료초액불대칭진양적실시가능성,이급대초액맥동성적보상조시。탑건료류식평태진행형광미구적CV치측정,기변화추세여방진결과교위문합。방진결과위류식세포의액류계통적설계제공료삼고의거。
Simulated analysis on flow cell ,which is core components of fluid system in flow cytometer , was conducted by use of FLUENT. The influence of sample flow rate ,sheath flow rate ,sample outlet po-sition on the focusing capability were discussed. The asymmetrical sheath inlet was also investigated ,as well as the compensation of sheath pulsation. The experimental platform was built to test the CV of fluo-rescent microspheres. The changing of CV coincided with the simulated results. The simulated results provide a reference to the design of fluid system in flow cytometer.