CAJ | 학술논문

目的：建立栀子金花分散片中盐酸小檗碱的含量测定方法。方法：采用HPLC法测定，色谱条件：Diamonsil-C18色谱柱(4.6mm×150mm，5μm)，流动相为乙腈-0.6%磷酸二氢钠溶液(30：70)，流速为1.0ml/min，检测波长为350nm。结果：盐酸小檗碱在2.5-50μg范围内线性关系良好，r=0.9995；盐酸小檗碱加样回收率(n=6)为100.85%，RSD为3.7%；栀子金花分散片中盐酸小檗碱平均含量为0.633%。结论：本研究建立的HPLC测定栀子金花分散片中盐酸小檗碱含量的方法简便、重复性好，可用于栀子金花分散片中盐酸小檗碱的含量测定。
목적：건립치자금화분산편중염산소벽감적함량측정방법。방법：채용HPLC법측정，색보조건：Diamonsil-C18색보주(4.6mm×150mm，5μm)，류동상위을정-0.6%린산이경납용액(30：70)，류속위1.0ml/min，검측파장위350nm。결과：염산소벽감재2.5-50μg범위내선성관계량호，r=0.9995；염산소벽감가양회수솔(n=6)위100.85%，RSD위3.7%；치자금화분산편중염산소벽감평균함량위0.633%。결론：본연구건립적HPLC측정치자금화분산편중염산소벽감함량적방법간편、중복성호，가용우치자금화분산편중염산소벽감적함량측정。
Objective:To establish a method for determining of berberine hydrochloride content in ZhiziJinhua Dispersible Tablet.Methods:HPLC was used to determine berberine hydrochloride content in ZhiziJinhua Dispersible Tablet. The chromatographic column was Diamonsil-C18 (4.6×150mm, 5μm), with acetonitrile-0.6%NaH2PO4 (30:70) as mobile phase, flow rate:1.0ml/min, detection wavelength:350nm. Results:The linear range of berberine hydrochloride was 2.5-50μg (r=0.9995);the average recovery of berberine hydrochloride was 100.85%, RSD was3.7%(n=6). The average content of berberine hydrochloride in ZhiziJinhua Dispersible Tablet was 0.633%.Conclusions:Determination of berberine hydrochloride content in ZhiziJinhua Dispersible Tablet by HPLC in this study is simple and reproducible, and can be used for determiningberberinehydrochloridecontentin ZhiziJinhua DispersibleTablet.