CAJ | 학술논문

目的：探讨高糖、高胰岛素和不同浓度二甲双胍对乳腺癌MCF-7细胞增殖的影响。方法：体外培养人乳腺癌细胞株MCF-7，分别给予高糖和(或)高胰岛素及不同浓度的二甲双胍进行干预，MTT法检测MCF-7细胞的增殖情况。结果：高糖、高胰岛素、高糖高胰岛素作用不同时间均可促进MCF-7细胞增殖；不同浓度的二甲双胍干预后，MCF-7细胞的生长均受到一定程度的抑制，且随时间和浓度的增加而增加。结论：不同浓度二甲双胍可对高糖、高胰岛素作用下乳腺癌MCF-7细胞的增殖起到抑制作用，且呈时间和浓度依赖性。
목적：탐토고당、고이도소화불동농도이갑쌍고대유선암MCF-7세포증식적영향。방법：체외배양인유선암세포주MCF-7，분별급여고당화(혹)고이도소급불동농도적이갑쌍고진행간예，MTT법검측MCF-7세포적증식정황。결과：고당、고이도소、고당고이도소작용불동시간균가촉진MCF-7세포증식；불동농도적이갑쌍고간예후，MCF-7세포적생장균수도일정정도적억제，차수시간화농도적증가이증가。결론：불동농도이갑쌍고가대고당、고이도소작용하유선암MCF-7세포적증식기도억제작용，차정시간화농도의뢰성。
Objective:To investigate the effects of high glucose, high insulin and different concentration metformin on proliferation of breast cancer cell line MCF-7.Methods:MTT method was used to detect the proliferation of MCF-7 cells after the MCF-7 cells were intervened by high glucose and/or high insulin and different concentration of metformin in vivo. Results:Highglucoseand/orhighinsulincouldpromoteproliferationofMCF-7cellsatdifferentactiontime.Afterintervened bydifferentconcentrationofmetformin,thegrowth ofMCF-7 was inhibited to acertain extentwhich increased with timeand concentration.Conclusions:Different concentration of metformin can inhibit proliferation of MCF-7 cells under the action of highglucoseandhighinsulin,whichshows timeand the concentration dependence.