CAJ | 학술논문

采用半静水生物测试法，研究亚硝酸盐氮对红鳍东方鲀（Takifugu rubripes）（9.5~9.8 g）的急性毒性及对部分肝脏抗氧化酶活性的影响。结果表明，在急性毒性实验中，亚硝酸盐氮对红鳍东方鲀的24、48、72和96 h半致死质量浓度（LC50）分别为201.88、190.57、171.09、159.25 mg/L，安全质量浓度为15.92 mg/L；在亚急性毒性实验中，当红鳍东方鲀分别在5.3、10.6，15.9、21.2 mg/L亚硝酸盐氮中暴露4周时，其肝脏总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性随亚硝酸盐氮浓度的升高均被显著抑制，差异有统计学意义（P<0.05），表明亚硝酸盐氮会对鱼体的抗氧化能力产生影响。
채용반정수생물측시법，연구아초산염담대홍기동방돈（Takifugu rubripes）（9.5~9.8 g）적급성독성급대부분간장항양화매활성적영향。결과표명，재급성독성실험중，아초산염담대홍기동방돈적24、48、72화96 h반치사질량농도（LC50）분별위201.88、190.57、171.09、159.25 mg/L，안전질량농도위15.92 mg/L；재아급성독성실험중，당홍기동방돈분별재5.3、10.6，15.9、21.2 mg/L아초산염담중폭로4주시，기간장총항양화능력（T-AOC）、초양화물기화매（SOD）화과양화경매（CAT）활성수아초산염담농도적승고균피현저억제，차이유통계학의의（P<0.05），표명아초산염담회대어체적항양화능력산생영향。
The acute toxicity of nitrite to Takifugu rubripes was studied by semi-static bioassay method at the conditions of pH 8.0, DO 7 mg/L, salinity 32 and water temperature 24℃. The results showed that the 24, 48, 72, and 96 h median lethal concentrations (LC50) of nitrite-N to Takifugu rubripes were 201.88, 190.57, 171.09, and 159.25 mg/L respectively, as well as the safe concentration was 15.92 mg/L. The subacute experimental concentrations of nitrite were set at 5.3, 10.6, 15.9, 21.2mg/L, and the exposure duration was 4 weeks, and the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT) activity in liver were used as an indicator of nitrite stress on toxicological effect. The results of sub acute experiment showed that the activities of T-AOC SOD and CAT in the liver were restrained with statistical significance (P<0.05), indicating that the nitrite had toxic effects on Takifugu rubripes.