CAJ | 학술논문

目的：观察原位注射新生大鼠肝细胞构建的工程化肝组织对实验性肝纤维化大鼠血清和肝组织学的影响，探讨其抗肝纤维化的作用。方法用四氯化碳制成实验性肝纤维化造模大鼠，肝脏原位多点注射工程化肝组织，3周后测定血清肝功能并行肝组织学检查。结果原位注射组大鼠肝功能较单纯假手术组大鼠有改善，且具有显著差异(P<0.05)，HE染色提示纤维化明显改善。结论肝脏原位多点注射新生大鼠肝细胞的工程化肝组织能改善大鼠肝纤维化进程，有一定抗肝纤维化作用。
목적：관찰원위주사신생대서간세포구건적공정화간조직대실험성간섬유화대서혈청화간조직학적영향，탐토기항간섬유화적작용。방법용사록화탄제성실험성간섬유화조모대서，간장원위다점주사공정화간조직，3주후측정혈청간공능병행간조직학검사。결과원위주사조대서간공능교단순가수술조대서유개선，차구유현저차이(P<0.05)，HE염색제시섬유화명현개선。결론간장원위다점주사신생대서간세포적공정화간조직능개선대서간섬유화진정，유일정항간섬유화작용。
Objective To observe the effecting on the content of liver function in serum and liver fibrosis index of experimental liver fibrogenesis rats,and investigate wether injection of hepatic constructs based on neonatal rat is provided with anti-liver fibrogenesis effect. Methods The rats were divided into two groups,and in group one,engineered hepatic grafts were implanted by orthotopically multi-spot injection. Liver cells isolated from from neonatal SD rats were mixed with liquid collagen prepared from SD rat tails.While group two received the same volume of saline solution.Liver function was evaluated by serum biological analysis at 10days. After 20days rats were killed,fibrosis index was examined by HE staining and Sirius red staining of liver tissue. Results Compared to controls,injection of hepatic constructs based on neonatal rat improve the liver function. Moreover,fibrosis index got more improvement in cell-matrix mixture than that in control group. Conclusion Multi-site injection of hepatic constructs based on neonatal rat liver cells in the liver can protect against experimental liver fibrosis in CCl4-induced rats and the mechanisms will be studied further.