CAJ | 학술논문

目的：研究致婴幼儿败血症猪霍乱沙门菌的耐药基因特点及同源性。方法16株猪霍乱沙门菌，采用K-B纸片扩散法检测抗菌药物的耐药性，同时用PCR法及DNA测序法对其进行β-内酰胺酶类耐药基因检测；PFGE法测定同源性。结果对氨苄西林耐药的13株猪霍乱沙门菌经PCR扩增后并测序为blaTEM-1，16株猪霍乱沙门菌经PFGE分型，可分为5个PFGE型，其中A型是最主要的克隆(12/16)。13株blaTEM-1基因阳性菌株分别为A型11株，B型1株，D型1株。结论产TEM-1型β-内酰胺酶是本地区猪霍乱沙门菌对氨苄西林耐药的主要机制，PFGE分型方法对猪霍乱沙门菌分型能力较好，克隆性传播为猪霍乱沙门菌的主要传播途径，同一PFGE型菌株的耐药谱非常接近。
목적：연구치영유인패혈증저곽란사문균적내약기인특점급동원성。방법16주저곽란사문균，채용K-B지편확산법검측항균약물적내약성，동시용PCR법급DNA측서법대기진행β-내선알매류내약기인검측；PFGE법측정동원성。결과대안변서림내약적13주저곽란사문균경PCR확증후병측서위blaTEM-1，16주저곽란사문균경PFGE분형，가분위5개PFGE형，기중A형시최주요적극륭(12/16)。13주blaTEM-1기인양성균주분별위A형11주，B형1주，D형1주。결론산TEM-1형β-내선알매시본지구저곽란사문균대안변서림내약적주요궤제，PFGE분형방법대저곽란사문균분형능력교호，극륭성전파위저곽란사문균적주요전파도경，동일PFGE형균주적내약보비상접근。
Objective To investigate drug-resistant genes and homology of Salmonella choleraesuis isolates from the blood of children with septicemia. Methods A total of 16 strains of Salmonella choleraesuis were collected from children with septicemia. Antimicrobial susceptibility was determined by K-B disk,PCR and DNA sequencing were used for detecting β-lactamases and quinolones genes. Their homology was identified by PFGE. Results Thirteen strains of ampicillin resistant Salmonella choleraesuis were identified by PCR amplifying and DNA Sequencing and their drug-resistant gene was bla TEM-1. 16 strains of Salmonella choleraesuis were classified into 9 PFGE types. A type was the most important clone (12/16). Thirteen strains with bla TEM-1 gene in-clude A type(11 strains), B type(1 strains) and D type(1 strains). Conclusion For Salmonella choleraesuis, β-lactamases based on TEM-1 gene is the primary ampicillin resistant mechanism. PFGE is a proper method for Salmonella choleraesuis classification and the strains from same PFGE type have similar drug resistance spectrum. And clonal spread is the main transmission route for Salmonella choleraesuis.