CAJ | 학술논문

目的：初步确立软骨细胞的二维生物打印方法，实现对细胞喷射过程的控制并保持打印后的细胞活力。方法取原代软骨细胞，常规培养至第2代。实验分2组：打印组，快速成型组织打印机进行二维细胞打印，X轴间隔300μm， Y轴间隔1500μm，激光共聚焦显微镜观察，经生物打印后培养2 h，Live/Dead viability Kit测定细胞活力，激光共聚焦显微镜观察细胞荧光染色情况；对照组除细胞悬液不行打印，其余操作同打印组。结果打印组细胞激光共聚焦显微镜观察，“细胞墨滴”在二维组织中均匀分布，满足二维设计细胞打印的要求，每个“细胞墨滴”含细胞15~35个。细胞活力测试显示，打印组细胞活力与对照组无明显区别。结论通过生物打印技术可实现软骨细胞在二维平面上的定向、定量规则分布，为进一步的细胞三维打印乃至器官打印体系奠定基础。
목적：초보학립연골세포적이유생물타인방법，실현대세포분사과정적공제병보지타인후적세포활력。방법취원대연골세포，상규배양지제2대。실험분2조：타인조，쾌속성형조직타인궤진행이유세포타인，X축간격300μm， Y축간격1500μm，격광공취초현미경관찰，경생물타인후배양2 h，Live/Dead viability Kit측정세포활력，격광공취초현미경관찰세포형광염색정황；대조조제세포현액불행타인，기여조작동타인조。결과타인조세포격광공취초현미경관찰，“세포묵적”재이유조직중균균분포，만족이유설계세포타인적요구，매개“세포묵적”함세포15~35개。세포활력측시현시，타인조세포활력여대조조무명현구별。결론통과생물타인기술가실현연골세포재이유평면상적정향、정량규칙분포，위진일보적세포삼유타인내지기관타인체계전정기출。
Objective To establish a two-dimensional biological printing technique of chondrocytes so as to control the cell transfer process and keep cell viability after printing. Methods Primary chondrocytes were obtained from auricles of 8-week-old piglets and then were regularly sub-cultured to passage 2 (P2). The experiment was divided into 2 groups:printing group and control group. In printing group, P2 chondrocytes were transferred by rapid prototype biological printer (interval in x-axis 300 μm, interval in y-axis 1 500 μm), and were then cultured for 2 hours, afterwards cell viability was detected by Live/Dead viability Kit and cell fluorescence was observed by laser scanning confocal microscope; In control group, all steps were the same as printing group except that cell suspension received no printing. Results Laser scanning confocal microscope observation on the cells in printing group revealed the “cell ink droplets”. They were distributed regularly and evenly in the two-dimensional layer and each contained 15-35 cells, meeting the requirement of designing two-dimensional cell printing. The cells in printing group went through cell viability test, laser scanning confocal microscope observation showed that it was no significant difference between the control group and the printing groups in terms of cell viability. Conclusion Biological printing technique can realize the oriented, quantificational and regular distribution of chondrocytes in the two-dimensional plane and lays the foundation for the construction of three-dimensional cell printing or even organ printing system.