CAJ | 학술논문

通过阀切换并联式双系统，建立了一套测定土壤样品中7种芳磺酸的反相离子对液相色谱法。该方法采用2根色谱柱，一根色谱柱分析的同时，另一根色谱柱通过阀切换实现平衡，节约了38?5％的分析时间。方法使用 Dikma Diamonsil C18（150 mm ×4?6 mm ×5μm）色谱柱，以离子对试剂（四丁基硫酸氢铵）和乙腈为流动相，检测波长为226、235、258 nm。各组分线性关系良好，线性相关系数均在0?9999以上。随机抽取样品进行重新提取、检测，得到各组分的相对标准偏差均小于7％，且定量限最高不超过0?1812μg/g，重复性和定量限均能满足实验要求。
통과벌절환병련식쌍계통，건립료일투측정토양양품중7충방광산적반상리자대액상색보법。해방법채용2근색보주，일근색보주분석적동시，령일근색보주통과벌절환실현평형，절약료38?5％적분석시간。방법사용 Dikma Diamonsil C18（150 mm ×4?6 mm ×5μm）색보주，이리자대시제（사정기류산경안）화을정위류동상，검측파장위226、235、258 nm。각조분선성관계량호，선성상관계수균재0?9999이상。수궤추취양품진행중신제취、검측，득도각조분적상대표준편차균소우7％，차정량한최고불초과0?1812μg/g，중복성화정량한균능만족실험요구。
Established a method for extraction and fast detection of 7 kinds of aromatic sulfonic acid ( anthraquinone 1, 5-disulfonic acid, 1-amino-8-naphthol-3, 6-disulfonic acid, 1-naphthol-4-sulfonic, anthraquinone-1, 8-disulfonic acid, 1-naphthalene sulfonic acid, 2-naphthalene sulfonic acid, 3-hydroxy-2-naphthoic acid) in soil by valve switching Ion-pair RP-HPLC with a dual system. Two columns were used in this system. When one was in the process of determination, the other one was in the process of equilibration. Thus 38?5% time was saved. Analytical columns were Dikma Diamonsil C18 (150 mm × 4?6 mm × 5 μm) , eluents were ion-pair reagent ( Tetrabutylammonium Hydrogen Sulfate ) and acetonitrile, and wave lengths were 226, 235, 258 nm. A good linear relationship was obtained for each aromatic sulfonic acid. All linear correlation coefficients were more than 0?999 9. Extracted and detected the randomly selected samples, and got each RSD of the 7 compounds less than 7% as well as the quantification limit no more than 0?181 2 μg/g. Both of the repeatability and quantification limit meet the requirements of detection.