CAJ | 학술논문

目的：研究林蛙油复方冲剂对X射线辐射所致大鼠氧化损伤能力的影响。方法 Wistar大鼠50只，随机分为5组：分别为空白对照组、单纯照射组、照射给药组（高、中、低剂量林蛙油灌胃），除空白对照组其余各组6 Gy全身一次性照射建立辐射模型，照射给药组分别按200、100、50 mg/（ kg· d）林蛙油进行灌胃，每天1次，连续灌胃7 d。称重后动物处理，从腹主动脉抽血对动物外周血及血清丙二醛（ MDA）含量和超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力进行检测。结果与空白对照组比较，单纯照射组大鼠的WBC、RBC数明显降低（P＜0．01），大鼠的MDA含量明显增高（P＜0．01），血清SOD、GSH-Px活力显著降低（ P＜0．01）；与单纯照射组比较，给药组大鼠的WBC、RBC数均明显增高，差异有统计学意义（ P＜0．01， P＜0．05）。大鼠MDA含量无明显差异（ P＞0．05），但有降低趋势；林蛙油100 mg组、林蛙油200 mg组的血清T-SOD、GSH-Px活力明显升高（ P＜0．01）。结论林蛙油复方冲剂可以通过拟制MDA含量，调节T-SOD、GSH-Px活力有效的提高机体的抗氧化能力，发挥对机体的保护作用。
목적：연구림와유복방충제대X사선복사소치대서양화손상능력적영향。방법 Wistar대서50지，수궤분위5조：분별위공백대조조、단순조사조、조사급약조（고、중、저제량림와유관위），제공백대조조기여각조6 Gy전신일차성조사건립복사모형，조사급약조분별안200、100、50 mg/（ kg· d）림와유진행관위，매천1차，련속관위7 d。칭중후동물처리，종복주동맥추혈대동물외주혈급혈청병이철（ MDA）함량화초양화물기화매（SOD）、곡광감태과양화물매（GSH-Px）적활력진행검측。결과여공백대조조비교，단순조사조대서적WBC、RBC수명현강저（P＜0．01），대서적MDA함량명현증고（P＜0．01），혈청SOD、GSH-Px활력현저강저（ P＜0．01）；여단순조사조비교，급약조대서적WBC、RBC수균명현증고，차이유통계학의의（ P＜0．01， P＜0．05）。대서MDA함량무명현차이（ P＞0．05），단유강저추세；림와유100 mg조、림와유200 mg조적혈청T-SOD、GSH-Px활력명현승고（ P＜0．01）。결론림와유복방충제가이통과의제MDA함량，조절T-SOD、GSH-Px활력유효적제고궤체적항양화능력，발휘대궤체적보호작용。
Objective To study the influence of X-rays radiation on the antioxidation in rats and the oviductus ranae's protection for the radiation damage .Methods Fifty Wistar rats were randomly divide into five groups:blank group,radiation model group,radiation medicine group (high,medium,low dose oviductus rana with gastric infusion ), 6 Gy radiation for all the body to made radiation model ,the radiation medicine group were 200,100,50 mg/(Kg· d), once time a day,continue 7 days.The content of malondialdehyde (MDA),superoxide dismutase (SOD) and the glu-tathione peroxidase ( GSH-Px) from the animal peripheral blood would be tested .Results Compared with the blank group,the radiation model rats reduced notably (P<0.01)in the quantity of the WBC and RBC.The content of the rats'MDA(P<0.01)increased notably,the vigor of the SOD,GSH-Px in the serum reduced notably(P<0.01).Com-pared with the purely radiated group ,the quantity of the WBC and RBC in the medicine group increased notably .There were statistical significance in difference (P<0.01,P<0.05).The content of the rats'MDA(P<0.01)had no obvi-ous difference ,but appeared a trend of being lower .The vigor of the SOD ,GSH-Px in the serum of the 100 mg,200 mg Rana japonica group has increased notably (P<0.01).Conclusion Compound recipe of Rana japonica oil have the antioxidation capacity and play a protective role for body .