CAJ | 학술논문

目的：探讨右美托咪定（Dex）对脊髓缺血再灌注损伤大鼠脊髓组织及血浆TNF-α和 IL-8表达的影响。方法成年雄性Wister大鼠54只，随机分为3组，假手术组、缺血再灌注组、缺血再灌注Dex处理组。Zivin改进法制作脊髓缺血再灌注模型。 Dex组于夹闭腹主动脉前30min 输注Dex 5μg/（kg? h）至再灌注后30min。同期内Sham组与I/R组输注等容量生理盐水。再灌注后6h，12h和24h 用酶联免疫吸附法和Lowry法分别测TNF-α和IL-8含量。结果①I/R组脊髓组织中TNF-α随再灌注时间延长而逐渐增高，各时间点明显高于Dex组（P＜0．05）。缺血再灌注24h后血浆TNF-α含量I/R组和Dex组均有增加，但后者明显低于I/R组（P＜0．05）。②各时间点脊髓组织I/R组IL-8含量较Sham组明显升高（P＜0．05），Dex组于再灌注12h和24h略高于Sham组，但明显低于I/R组（P＜0．05）。再灌注12h和24h I/R组血浆IL-8含量明显高于Sham组（P＜0．05），而Dex组各时间点均明显低于I/R组（P＜0．05）。结论Dex能够抑制大鼠脊髓缺血再灌注损伤脊髓组织及血浆TNF-α和IL-8的表达，对脊髓缺血再灌注损伤有一定的神经保护作用。
목적：탐토우미탁미정（Dex）대척수결혈재관주손상대서척수조직급혈장TNF-α화 IL-8표체적영향。방법성년웅성Wister대서54지，수궤분위3조，가수술조、결혈재관주조、결혈재관주Dex처리조。Zivin개진법제작척수결혈재관주모형。 Dex조우협폐복주동맥전30min 수주Dex 5μg/（kg? h）지재관주후30min。동기내Sham조여I/R조수주등용량생리염수。재관주후6h，12h화24h 용매련면역흡부법화Lowry법분별측TNF-α화IL-8함량。결과①I/R조척수조직중TNF-α수재관주시간연장이축점증고，각시간점명현고우Dex조（P＜0．05）。결혈재관주24h후혈장TNF-α함량I/R조화Dex조균유증가，단후자명현저우I/R조（P＜0．05）。②각시간점척수조직I/R조IL-8함량교Sham조명현승고（P＜0．05），Dex조우재관주12h화24h략고우Sham조，단명현저우I/R조（P＜0．05）。재관주12h화24h I/R조혈장IL-8함량명현고우Sham조（P＜0．05），이Dex조각시간점균명현저우I/R조（P＜0．05）。결론Dex능구억제대서척수결혈재관주손상척수조직급혈장TNF-α화IL-8적표체，대척수결혈재관주손상유일정적신경보호작용。
Objective To explore effects of dexmedetomidine (Dex) on the expression of TNF-αand IL-8 in plasma and mye-loid tissue after spinal cord ischemia/reperfusion in rats.Methods Fifty-four healthy male Wister rats weighing 200~260g were randomly divided into three groups:Sham operation group (Sham group,n=18),ischemia/reperfusion group(I/R group,n=18) and Dex treatment group(Dex group,n=18).The infusion of Dex 5μg/(kg? h)(Dex group) or normal saline(Sham group and I/R group) was given from the time of 30 minutes before occlusion of abdominal artery to 30 minutes after reperfusion.The TNF-αand IL-8 protein content was assessed at 6h,12h and 24h reperfusion.Results ①TNF-αin the spinal cord tissue In I/R group was significantly higher than that in Dex group at ev-ery time point(P<0.05).24h after reperfusion,plasma TNF-αlevels in I/R group and Dex group had increased ,but the latter was signifi-cantly lower than the I/R group(P<0.05).②Compared with Sham group,IL-8 level in spinal cord was significantly higher in I/R group, slightly higher at 12h,24h reperfusion in Dex group which was obviously lower than that in I /R group(P<0.05).The plasma IL-8 in I/R group was significantly higher than that in Sham group at 12h,24h reperfusion (P<0.05),while the Dex group were significantly lower than I/R group(P<0.05).Conclusion When the rat spinal cord ischemia-reperfusion injury occurred,Dex can inhibit the expression of TNF-αand IL-8 in spinal cord tissue and plasma,and has a effect of neuroprotective .