石河子大学学报(自然科学版)
石河子大學學報(自然科學版)
석하자대학학보(자연과학판)
JOURNAL OF SHIHEZI UNIVERSITY (NATURAL SCIENCE)
2014年
1期
69-72
,共4页
袁勇%邢建国%王立萍%姚佳茗%王新春
袁勇%邢建國%王立萍%姚佳茗%王新春
원용%형건국%왕립평%요가명%왕신춘
香青兰总黄酮%药代动力学%高效液相色谱法
香青蘭總黃酮%藥代動力學%高效液相色譜法
향청란총황동%약대동역학%고효액상색보법
Dracocephalum Moldavica L.Flavonoids%Pharmacokinetic%HPLC
为探讨香青兰总黄酮在大鼠体内的药代动力学。采用以田蓟苷为指标, HPLC法测定大鼠灌胃给药后血浆中田蓟苷的浓度,并采用DAS2.0软件计算药代动力学参数。结果显示,血浆中田蓟苷浓度在0.031~39.68μg/mL范围内线性关系良好(R=0.9990),日内精密度(RSD)<5%,日间精密度(RSD)<10%,方法回收率在98.01%~103.23%之间,提取回收率在72.67%~90.35%之间。香青兰总黄酮在大鼠体内呈二室分布,大鼠灌胃香青兰总黄酮提取物3个剂量(300,600,1200 mg/kg)后,t1/2β分别为(6.904±0.922),(6.512±3.302),(9.820±3.116)h,AUC (0-t)分别为(0.527±0.018),(0.980±0.097),(1.215±0.108) mg/L/h,Tmax分别为(0.292±0.083),(0.139±0.048),(0.222±0.048)h,Cmax分别为(0.177±0.018),((0.451±0.064),(0.656±0.115) mg/L。由此可知,该法适用于测定香青兰总黄酮在大鼠体内的血药浓度并进行药代动力学研究。
為探討香青蘭總黃酮在大鼠體內的藥代動力學。採用以田薊苷為指標, HPLC法測定大鼠灌胃給藥後血漿中田薊苷的濃度,併採用DAS2.0軟件計算藥代動力學參數。結果顯示,血漿中田薊苷濃度在0.031~39.68μg/mL範圍內線性關繫良好(R=0.9990),日內精密度(RSD)<5%,日間精密度(RSD)<10%,方法迴收率在98.01%~103.23%之間,提取迴收率在72.67%~90.35%之間。香青蘭總黃酮在大鼠體內呈二室分佈,大鼠灌胃香青蘭總黃酮提取物3箇劑量(300,600,1200 mg/kg)後,t1/2β分彆為(6.904±0.922),(6.512±3.302),(9.820±3.116)h,AUC (0-t)分彆為(0.527±0.018),(0.980±0.097),(1.215±0.108) mg/L/h,Tmax分彆為(0.292±0.083),(0.139±0.048),(0.222±0.048)h,Cmax分彆為(0.177±0.018),((0.451±0.064),(0.656±0.115) mg/L。由此可知,該法適用于測定香青蘭總黃酮在大鼠體內的血藥濃度併進行藥代動力學研究。
위탐토향청란총황동재대서체내적약대동역학。채용이전계감위지표, HPLC법측정대서관위급약후혈장중전계감적농도,병채용DAS2.0연건계산약대동역학삼수。결과현시,혈장중전계감농도재0.031~39.68μg/mL범위내선성관계량호(R=0.9990),일내정밀도(RSD)<5%,일간정밀도(RSD)<10%,방법회수솔재98.01%~103.23%지간,제취회수솔재72.67%~90.35%지간。향청란총황동재대서체내정이실분포,대서관위향청란총황동제취물3개제량(300,600,1200 mg/kg)후,t1/2β분별위(6.904±0.922),(6.512±3.302),(9.820±3.116)h,AUC (0-t)분별위(0.527±0.018),(0.980±0.097),(1.215±0.108) mg/L/h,Tmax분별위(0.292±0.083),(0.139±0.048),(0.222±0.048)h,Cmax분별위(0.177±0.018),((0.451±0.064),(0.656±0.115) mg/L。유차가지,해법괄용우측정향청란총황동재대서체내적혈약농도병진행약대동역학연구。
To study pharmacokinetics of Dracocephalum Moldavica L.Flavonoids in rat. Plasma concentration of tilianin was used as the indicators,which was measured by HPLC.The pharmacokinetic parameters were calculated by DAS2.0.It was showed good linear relationship when the concentration of tilianin in the range of 0.031~39.68 μg/mL with the correlation coefficient of 0.9990.The intra-day precision of this method was less than 5%,and the inter-day precision was less than 10%.The accuracy was 98.01%~103.23%.The extraction recovery was 72.67%~90.35%.The main pharmacokinetics parameters of tilianin after oral administration with three different doses (300,600,1200 mg/kg) to rats were: t1/2β:(6.904±0.922),(6.512±3.302),(9.820±3.116)h;AUC(0-t):(0.527±0.018),(0.980±0.097),(1.215±0.108) mg/L/h;Tmax:(0.292±0.083),(0.139±0.048),(0.222±0.048)h;Cmax:(0.177±0.018), (0.451 ±0.064), (0.656 ±0.115) mg/L.The method is proved to be suitable for the pharmacokinetic study of Dracocephalum Moldavica L.Flavonoids in rats.