CAJ | 학술논문

2012年在烤烟（Nicotiana tabacum L.）苗期发现茎部腐烂病，经病原菌的形态特征观察、rDNA-ITS和EF-1α序列分析，该致病菌被鉴定为九州镰孢菌（Fusarium kyushuense）。该病原菌在利马豆培养基上生长温度范围为5~41℃，最适30℃；分生孢子产生温度范围为10~35℃，最适35℃；分生孢子萌发温度范围为5~42℃，最适20~35℃；分生孢子萌发相对湿度范围为70%~100%，最适100%；可生长pH值为2~11，最适5~7；光照对菌丝生长和分生孢子萌发没有影响，光暗交替培养菌丝生长变慢，但分生孢子产量增加；在供试的95种碳源中，该病原菌能利用葡萄糖、麦芽糖、反丁烯二酸等89种碳源，不能利用N-乙酰基D-半乳糖胺、N-乙酰基-?-D-葡萄糖胺、葡萄醛酰胺、癸二酸、L-丝氨酸和腺苷-5’-磷酸盐6种碳源。
2012년재고연（Nicotiana tabacum L.）묘기발현경부부란병，경병원균적형태특정관찰、rDNA-ITS화EF-1α서렬분석，해치병균피감정위구주렴포균（Fusarium kyushuense）。해병원균재리마두배양기상생장온도범위위5~41℃，최괄30℃；분생포자산생온도범위위10~35℃，최괄35℃；분생포자맹발온도범위위5~42℃，최괄20~35℃；분생포자맹발상대습도범위위70%~100%，최괄100%；가생장pH치위2~11，최괄5~7；광조대균사생장화분생포자맹발몰유영향，광암교체배양균사생장변만，단분생포자산량증가；재공시적95충탄원중，해병원균능이용포도당、맥아당、반정희이산등89충탄원，불능이용N-을선기D-반유당알、N-을선기-?-D-포도당알、포도철선알、계이산、L-사안산화선감-5’-린산염6충탄원。
Stem rot in flue-cured tobacco (Nicotiana tabacum L.) was observed in tobacco seedlings in 2012. Its pathogen was identified as Fusarium kyushuense according to morphology, pathogenicity and rDNA-ITS and EF-1αgene analysis. The temperature for the pathogen to grow on LBA ranged from 5℃to 42℃with an optimum temperature of 30℃, ranged from 10℃to 35℃with optimum temperature of 35℃;and ranged from 5℃to 42℃with optimum temperature of 30℃to 35℃for conidial germination. The suitable relative humidity for conidial germination of pathogen ranged from 70%to 100%. The pathogen could grow under pH 2-11 with optimum pH 5-7. Light had no effect on mycelial growth and conidial germination. Light and dark alternation reduced mycelial growth, but enhanced sporulation. Among the 95 carbon resources in Biolog FF Microplate, 89 ones could be utilized by F. kyushuense. Carbon resources which could not be used by pathogen include N-acetyl-D-galactosamine, N-acetyl-?-D-glucosamine, glucuronamide, sebacic acid, L-serine and adenosine-5’-monophosphate.