CAJ | 학술논문

建立了苯酚提取超高效液相色谱法（UPLC）同时测定新鲜烟叶中三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和单磷酸腺苷（AMP）的分析方法。苯酚提取样品溶液中的ATP、ADP和AMP较稳定，不易水解，样品在2 h以内各磷酸腺苷变化率小于10%。采用6‰三乙胺（pH=6.6）和甲醇作为流动相梯度洗脱，3种磷酸腺苷在10 min内实现了较好的分离，线性范围（5～200）μg/mL内，磷酸腺苷浓度与峰面积相关性很高（r>0.9999），3种磷酸腺苷检测限均小于0.5μg/mL，回收率在91.68%～110.79%之间， RSD在1.53%～3.67%之间。
건립료분분제취초고효액상색보법（UPLC）동시측정신선연협중삼린산선감（ATP）、이린산선감（ADP）화단린산선감（AMP）적분석방법。분분제취양품용액중적ATP、ADP화AMP교은정，불역수해，양품재2 h이내각린산선감변화솔소우10%。채용6‰삼을알（pH=6.6）화갑순작위류동상제도세탈，3충린산선감재10 min내실현료교호적분리，선성범위（5～200）μg/mL내，린산선감농도여봉면적상관성흔고（r>0.9999），3충린산선감검측한균소우0.5μg/mL，회수솔재91.68%～110.79%지간， RSD재1.53%～3.67%지간。
A analytical method for simultaneous determination of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in fresh tobacco leaves was established by ultra high performance liquid chromatography (UPLC) with phenol extraction. ATP, ADP and AMP in phenol extraction were stable and difficult to be hydrolyzed with rate of change less than 10%within 2 hours. A better separation of ATP, ADP and AMP was achieved in 10 minutes by utilizing a gradient elution system of 6‰triethylamine (pH=6.6) and methanol as mobile phase. The linear range was between 5~200 μg.mL-1 with adenosine concentration and peak area were highly correlated (r>0.9999). The detection limits for ATP, ADP and AMP were 0.5 μg.mL-1, 0.4 μg.mL-1, 0.3 μg.mL-1 respectively, and average recovery rate was 91.68%~110.79%with RSD ranged between 1.53%~3.67%. This method can quickly and accurately detect ATP content in tobacco samples, thus providing important test indicators for the study of energy metabolism, especially tobacco CMS generation mechanism.