福建医科大学学报
福建醫科大學學報
복건의과대학학보
JOURNAL OF FUJIAN MEDICAL UNIVERSITY
2014年
4期
227-231
,共5页
吴春林%刘秀华%庄雪瑜%吴佩琴%许昭俊%许海刚%杨维群
吳春林%劉秀華%莊雪瑜%吳珮琴%許昭俊%許海剛%楊維群
오춘림%류수화%장설유%오패금%허소준%허해강%양유군
癌 ,鳞状细胞%钙黏着糖蛋白类%波形蛋白%淋巴转移%食管肿瘤%免疫组织化学
癌 ,鱗狀細胞%鈣黏著糖蛋白類%波形蛋白%淋巴轉移%食管腫瘤%免疫組織化學
암 ,린상세포%개점착당단백류%파형단백%림파전이%식관종류%면역조직화학
carcinoma,squamous cell%cadherins%vimentin%lymphatic metastasis%esophageal neoplasms%immunohistochemistry
目的：观察上皮间质转化相关标记物E-钙黏蛋白（E-cadherin）和Vimentin基因及其蛋白在食管鳞状细胞癌淋巴结转移组和非转移组中的表达，并分析E-cadherin和Vimentin表达的相关性。方法采用免疫组织化学SP法和实时定量逆转录聚合酶链反应（qRT-PCR）检测47例有淋巴结转移、30例无淋巴结转移的食管癌和20例正常食管组织中E-cadherin和Vimentin基因及其蛋白的表达情况。结果对照组、非转移组与转移组 E-cadherin蛋白表达分别为20/20（100％）、29/30（96．67％）和44/47（93．62％），组间比较差别有统计学意义（ P＜0．05）。转移组的高表达率22/47（46．8％）低于非转移组18/30（60％），但差别无统计学意义（ P＞0．05）。Vimentin蛋白在对照组、非转移组、转移组阳性表达率逐渐增高，分别为0/20（0）、11/30（36．67％）、37/47（78．72％），组间比较差别有统计学意义（ P＜0．05），转移组与非转移组间比较差别有统计学意义（ P＜0．05）。Vimentin和E-cadherin表达呈显著负相关，相关系数（ r＝-0．377）。qRT-PCR检测显示非转移组和转移组中E-cadherin和Vimentin mRNA表达量与对照组比较差别有统计学意义（P＜0．05）。E-cadherin基因的表达在转移组（0．43±0．23）与非转移组（0．51±0．54）间的差别无统计学意义（ P＞0．05），而转移组 Vimentin表达（4．05±1．88）明显高于非转移组（2．70±1．73），差别有统计学意义（ P＜0．05）。结论上皮间质转化相关基因E-cad-herin和Vimentin与食管鳞癌的转移密切相关，可作为预测食管癌转移复发及评估临床预后的有效标记物。
目的：觀察上皮間質轉化相關標記物E-鈣黏蛋白（E-cadherin）和Vimentin基因及其蛋白在食管鱗狀細胞癌淋巴結轉移組和非轉移組中的錶達，併分析E-cadherin和Vimentin錶達的相關性。方法採用免疫組織化學SP法和實時定量逆轉錄聚閤酶鏈反應（qRT-PCR）檢測47例有淋巴結轉移、30例無淋巴結轉移的食管癌和20例正常食管組織中E-cadherin和Vimentin基因及其蛋白的錶達情況。結果對照組、非轉移組與轉移組 E-cadherin蛋白錶達分彆為20/20（100％）、29/30（96．67％）和44/47（93．62％），組間比較差彆有統計學意義（ P＜0．05）。轉移組的高錶達率22/47（46．8％）低于非轉移組18/30（60％），但差彆無統計學意義（ P＞0．05）。Vimentin蛋白在對照組、非轉移組、轉移組暘性錶達率逐漸增高，分彆為0/20（0）、11/30（36．67％）、37/47（78．72％），組間比較差彆有統計學意義（ P＜0．05），轉移組與非轉移組間比較差彆有統計學意義（ P＜0．05）。Vimentin和E-cadherin錶達呈顯著負相關，相關繫數（ r＝-0．377）。qRT-PCR檢測顯示非轉移組和轉移組中E-cadherin和Vimentin mRNA錶達量與對照組比較差彆有統計學意義（P＜0．05）。E-cadherin基因的錶達在轉移組（0．43±0．23）與非轉移組（0．51±0．54）間的差彆無統計學意義（ P＞0．05），而轉移組 Vimentin錶達（4．05±1．88）明顯高于非轉移組（2．70±1．73），差彆有統計學意義（ P＜0．05）。結論上皮間質轉化相關基因E-cad-herin和Vimentin與食管鱗癌的轉移密切相關，可作為預測食管癌轉移複髮及評估臨床預後的有效標記物。
목적：관찰상피간질전화상관표기물E-개점단백（E-cadherin）화Vimentin기인급기단백재식관린상세포암림파결전이조화비전이조중적표체，병분석E-cadherin화Vimentin표체적상관성。방법채용면역조직화학SP법화실시정량역전록취합매련반응（qRT-PCR）검측47례유림파결전이、30례무림파결전이적식관암화20례정상식관조직중E-cadherin화Vimentin기인급기단백적표체정황。결과대조조、비전이조여전이조 E-cadherin단백표체분별위20/20（100％）、29/30（96．67％）화44/47（93．62％），조간비교차별유통계학의의（ P＜0．05）。전이조적고표체솔22/47（46．8％）저우비전이조18/30（60％），단차별무통계학의의（ P＞0．05）。Vimentin단백재대조조、비전이조、전이조양성표체솔축점증고，분별위0/20（0）、11/30（36．67％）、37/47（78．72％），조간비교차별유통계학의의（ P＜0．05），전이조여비전이조간비교차별유통계학의의（ P＜0．05）。Vimentin화E-cadherin표체정현저부상관，상관계수（ r＝-0．377）。qRT-PCR검측현시비전이조화전이조중E-cadherin화Vimentin mRNA표체량여대조조비교차별유통계학의의（P＜0．05）。E-cadherin기인적표체재전이조（0．43±0．23）여비전이조（0．51±0．54）간적차별무통계학의의（ P＞0．05），이전이조 Vimentin표체（4．05±1．88）명현고우비전이조（2．70±1．73），차별유통계학의의（ P＜0．05）。결론상피간질전화상관기인E-cad-herin화Vimentin여식관린암적전이밀절상관，가작위예측식관암전이복발급평고림상예후적유효표기물。
Objective To identify the differences of epithelial mesenchymal transition (EM T ) re-lated biomarkers of E-cadherin and Vimentin in mRNA and protein expression levels between esophageal squamous cell carcinoma (ESCC) with lymph node metastasis and no lymph node metastasis . Methods Immunohistochemistry SP method and quantitative reverse transcriptase real-time polymerase chain reac-tion (qRT-PCR) array were applied to detect mRNA and protein expression levels of E-cadherin and Vim-entin on 47 cases of ESCC with lymph node metastasis and 30 cases of ESCC without lymph node metasta-sis ,and 20 cases of normal esophageal mucous membrane tissues . Results The percentage of expressing E-cadherin in normal control group ,non metastasis and metastasis group of ESCC were 20/20 (100% ) , 29/30 (96 .67% ) ,and 44/47 (93 .62% ) respectively ,there was a statistically significant difference among three groups ( P<0 .05) ,but the percentage of high E-cadherin expression of metastasis group (22/47 , 46 .8% ) and non metastasis group (18/30 ,60% ) had no significant difference (P>0 .05) . The number of cases with the positive Vimentin protein expression increased in order of normal control group ,no me-tastasis group and metastasis group ,the percentage was (0/20 ,0) ,(11/30 ,36 .67% ) ,(37/47 ,78 .72% ) respectively ,showing a significant difference between two groups (P<0 .05) . The rate with high ex-pression level of Vimentin between the two groups of metastasis group (11/47 ,23 .4% ) and no metastasis (2/19 ,10 .53% ) had statistical significance (P<0 .05) . There was a significant negative correlation be-tween Vimentin and E-cadherin ,the correlation coefficient was r= -0 .377 . qRT-PCR analysis showed that there was a obvious difference in mRNA expression levels of E-cadherin and Vimentin in the normal group ,no metastasis group and metastasis group of ESCC (P< 0 .05) . E-cadherin mRNA expression level showed no significant difference between metastasis group (0 .43 ± 0 .23) and non metastasis group (0 .51 ± 0 .54)(P> 0 .05) ,while that of Vimentin had a significant difference between metastasis group (4 .05 ± 1 .88) and non metastasis group (2 .70 ± 1 .73) (P< 0 .05) . Conclusions The expressions of EM T related genes E-cadherin and Vimentin were closely correlated with metastasis of ESCC ,and there-fore could be used for predictors of the metastasis and recurrence of ESCC as well as useful biomarkers to prognosis .
