CAJ | 학술논문

目的：探讨胰高血糖素样肽-1（GLP-1）对高糖培养的原代人脐静脉内皮细胞（HUVECs）氧化应激情况的影响。方法体外分离、培养原代人 HUVECs ，分为对照组、高糖组（30 mmol/L ）及 GLP-1预处理组（GLP-1100 nmol/L预处理1 h后30 mmol/L高糖培养）。检测细胞上清乳酸脱氢酶（LDH）漏出量，进行细胞毒性作用定量分析；化学发光法检测细胞内活性氧族（ROS ）水平、黄嘌呤氧化酶法检测超氧化物歧化酶（SOD ）活力了解细胞内氧化应激状态。结果（1）GLP-1可以改善高糖环境对人脐静脉内皮细胞的毒性作用，高糖组LDH漏出量为对照组的1．8倍，GLP-1预处理组的LDH漏出量为对照组的1．3倍（P＜0．05）。（2）高糖组细胞内ROS荧光强度较对照组增强1倍以上，而GLP-1预处理组ROS荧光强度可抑制至对照组水平（P＜0．05）；与对照组比较，高糖组细胞内SOD酶活性无明显改变；但与高糖组比较，GLP-1预处理组SOD酶活性升高了64．64％（P＜0．05）。结论 GLP-1可以改善高糖对 HUVECs的细胞毒性作用，其机制可能是通过减轻细胞氧化应激来实现的。
목적：탐토이고혈당소양태-1（GLP-1）대고당배양적원대인제정맥내피세포（HUVECs）양화응격정황적영향。방법체외분리、배양원대인 HUVECs ，분위대조조、고당조（30 mmol/L ）급 GLP-1예처리조（GLP-1100 nmol/L예처리1 h후30 mmol/L고당배양）。검측세포상청유산탈경매（LDH）루출량，진행세포독성작용정량분석；화학발광법검측세포내활성양족（ROS ）수평、황표령양화매법검측초양화물기화매（SOD ）활력료해세포내양화응격상태。결과（1）GLP-1가이개선고당배경대인제정맥내피세포적독성작용，고당조LDH루출량위대조조적1．8배，GLP-1예처리조적LDH루출량위대조조적1．3배（P＜0．05）。（2）고당조세포내ROS형광강도교대조조증강1배이상，이GLP-1예처리조ROS형광강도가억제지대조조수평（P＜0．05）；여대조조비교，고당조세포내SOD매활성무명현개변；단여고당조비교，GLP-1예처리조SOD매활성승고료64．64％（P＜0．05）。결론 GLP-1가이개선고당대 HUVECs적세포독성작용，기궤제가능시통과감경세포양화응격래실현적。
Objective To investigate the effects and mechanism of glucagon-like peptide-1 (GLP-1) on high glucose-induced human umbilical vein endothelial cells (HUVECs) oxidative stress . Methods HUVECs was isolated and cultured in vitro ,randomly divided into control group ,high glucose (30 mmol/L) group and GLP-1 group (100 nmol/L GLP-1+ high glucose) . Endothelial toxicity was evaluated by lactate dehydrogenase (LDH) method . Intracellular reactive oxygen species (ROS) produc-tion and activity of superoxide dismutase (SOD) reflects the oxidative stress state in the cell . Fluores-cence enzyme-labeled instrument was used to detect the production of ROS . The activity of SOD was de-tected by xanthine oxidase method . Results (1)GLP-1 could reduce toxicity of high glucose . After high glucose trestment ,the ratio of LDH leakage was markly increased to 1 .8 times of the control group . <br> However ,the ratio of LDH leakage in cells pre-treated with 100 nmol/L GLP-1 significantly decreased to 1 .3 times of the control group(P<0 .05) . (2)Comparing with control group ,ROS production was in-creased more than 1 times(P<0 .05) and the activity of SOD had no significant changes in high glucose group . After GLP-1 pretreatment ,ROS was lowered to the level of control group and activity of SOD was increased by 64 .64% (P< 0 .05) . Conclusions GLP-1 has a direct protective effect on HUVECs function under high glucose condition ,w hich is probably related to the mitigation of oxidative stress .