CAJ | 학술논문

通过发病情况调查、临床症状观察、病理学检查及 AGP 和 PCR 诊断技术，对甘肃省康乐县某鸡场散养育成‘青脚麻鸡’群发生的疑似马立克病进行了诊断和病理学研究。结果表明：鸡群生长到8周龄开始发病、死亡，表现出典型的马立克病临床症状；病理剖检主要表现为除肺脏外大部分内脏实质器官产生大小不等形状不规则的肿瘤结节；病理组织学变化为各组织器官可见弥漫性的淋巴细胞浸润，但肿瘤细胞的异形性不明显；AGP试验为阳性；特异性PCR检测扩增出约804 bp的特异性meq基因片段，符合预期的片段大小。应用上述诊断技术确诊该鸡场‘青脚麻鸡’群暴发的是急性内脏型马立克病。
통과발병정황조사、림상증상관찰、병이학검사급 AGP 화 PCR 진단기술，대감숙성강악현모계장산양육성‘청각마계’군발생적의사마립극병진행료진단화병이학연구。결과표명：계군생장도8주령개시발병、사망，표현출전형적마립극병림상증상；병리부검주요표현위제폐장외대부분내장실질기관산생대소불등형상불규칙적종류결절；병리조직학변화위각조직기관가견미만성적림파세포침윤，단종류세포적이형성불명현；AGP시험위양성；특이성PCR검측확증출약804 bp적특이성meq기인편단，부합예기적편단대소。응용상술진단기술학진해계장‘청각마계’군폭발적시급성내장형마립극병。
In order to diagnose and pathological study of suspected of MD in the ‘green feet rearing chicken’backyard type in Kangle County of Gansu Provice,the sick chickens were studied by investigating the incidence,clinical observation,pathological examination and PCR diagnostic techniques.The results showed that chickens died morbidity,mortality when they grown to 8 weeks of age,and showed a typical clinical symptoms;the main manifestations of pathological examination was that most of solid organ except the lungs produces most visceral sizes irregularly shaped tumor nodules;histopathological changes of the tissues were that the organs had diffuse lymphocytic infiltration,but the tumor cell atypia was not obvious;AGP test was positive;the result of PCR showed that the amplified gene fragment of about 804 bp specific meq was the same with the expected fragment size.The application of the above diagnosis confirmed that the farm outbreak was acute visceral MD.