CAJ | 학술논문

目的：通过多种方法从大量天然中草药中筛选出一组具有防晒祛色素作用的中草药，为色素增加性皮肤病的治疗提供一新途径。方法：首先联合中医古籍和现代文献筛选出一组可能具有防晒祛色素作用的中草药，然后应用紫外分光光度法在200 nm~400 nm波长内扫描药物UVA和UVB段紫外吸光度值、蘑菇酪氨酸酶活性抑制法在酶标仪490 nm波长处检测产物吸光度值（A490）和DPPH自由基清除法测定药物DPPH清除率和DPPH半清除率（IC50）。结果：从中医古籍和现代文献筛选出人参、当归、茯苓、川芎、防风、白术、苡仁、桔梗、槐花、芦荟、黄芩、白芨、栀子、白芷、黄芪共15味中草药。其中槐花、芦荟、白芷UVA吸光度高于对照组（P＜0.01），黄芩、槐花、芦荟UVB吸光度高于对照组（P＜0.05）；芦荟、白术、槐花、茯苓、白芨和人参的酪氨酸酶抑制率较高，而芦荟和白术酪氨酸酶抑制作用较对照组强（P＜0.05）；上述中药对DPPH均有不同程度的清除作用，槐花、黄芩DPPH清除率和IC50接近于对照组（P >0.05）。最终筛选出人参、茯苓、白术、苡仁、桔梗、黄芪、黄芩、槐花、芦荟和白芨10味中草药。结论：本实验筛选出的10味中草药具有广谱紫外线吸收功能、抑制酪氨酸酶活性和抗自由基氧化作用，可为进一步色素增加性皮肤病的预防和治疗实验奠定重要基础。
목적：통과다충방법종대량천연중초약중사선출일조구유방쇄거색소작용적중초약，위색소증가성피부병적치료제공일신도경。방법：수선연합중의고적화현대문헌사선출일조가능구유방쇄거색소작용적중초약，연후응용자외분광광도법재200 nm~400 nm파장내소묘약물UVA화UVB단자외흡광도치、마고락안산매활성억제법재매표의490 nm파장처검측산물흡광도치（A490）화DPPH자유기청제법측정약물DPPH청제솔화DPPH반청제솔（IC50）。결과：종중의고적화현대문헌사선출인삼、당귀、복령、천궁、방풍、백술、이인、길경、괴화、호회、황금、백급、치자、백지、황기공15미중초약。기중괴화、호회、백지UVA흡광도고우대조조（P＜0.01），황금、괴화、호회UVB흡광도고우대조조（P＜0.05）；호회、백술、괴화、복령、백급화인삼적락안산매억제솔교고，이호회화백술락안산매억제작용교대조조강（P＜0.05）；상술중약대DPPH균유불동정도적청제작용，괴화、황금DPPH청제솔화IC50접근우대조조（P >0.05）。최종사선출인삼、복령、백술、이인、길경、황기、황금、괴화、호회화백급10미중초약。결론：본실험사선출적10미중초약구유엄보자외선흡수공능、억제락안산매활성화항자유기양화작용，가위진일보색소증가성피부병적예방화치료실험전정중요기출。
Objective:To screen herbs with sunscreening and depigmenting effect from a large number of natural plants or herbs for providing new therapy of skin hyperpigmentation disorders. Methods: Firstly, ancient Chinese medical and contemporary literatures were reviewed to pick out herbs which may have depigmenting potential. Then, these herbs were screened by UV spectrophotometry 200 nm~400 nm wavelength scaning to determine UVA and UVB absorption, mushroom tyrosinase inhibitory activities assay to detect to detect the absorption value (A490) by a microplate reader at 490 nm wavelength,and DPPH radical scavenging method to detect DPPH clearance and DPPH half clearance (IC50). Results:From ancient and modern literatures, 15 herbs were picked out.Among these herbs, the UVA absorption values of flos sophorae, aloes and angelica dahurica benth increased significantly compared with those of control group (P<0.01), while the UVB ones of scutellaria baicalensis georgi, flos sophorae and aloes remarkably excelled in those of control group (P < 0.05). Meanwhile, the tyrosinase inhibition rate of aloes, atractylodes macrocephala koidz, flos sophorae, tuckahoe, rhizoma bletillae striatae and ginseng ascent moderately, especially that of aloes and atractylodes macrocephala koidz greatly outweighed the control group (P<0.05). In addition, the above-mentioned herbs played roles of DPPH scavenging on various degrees, among which the DPPH clearance and IC50 of flos sophorae and scutellaria baicalensis georgi were close to those of control group (P> 0.05). Eventually, 10 herbs were selected:ginseng, tuckahoe, atractylodes macrocephala koidz, semen coicis, platycodon grandiflorum, radix astragali mongolici, scutellaria baicalensis georgi, flos sophorae, aloes and rhizoma bletillae striatae. Conclusions: These 10 herbs have broad-spectrum UV absorption, tyrosinase activity inhibiton, and anti-free radical oxidation, which may lay an important foundation for further experiments of treating hyperpigmentation dermatoses.