泸州医学院学报
瀘州醫學院學報
로주의학원학보
JOURNAL OF LUZHOU MEDICAL COLLEGE
2014年
1期
47-51
,共5页
杨思进%王承刚%白雪%罗钢%潘洪
楊思進%王承剛%白雪%囉鋼%潘洪
양사진%왕승강%백설%라강%반홍
高血压颈动脉粥样硬化%NF-κB%ICAM-1%蛭龙活血通瘀胶囊
高血壓頸動脈粥樣硬化%NF-κB%ICAM-1%蛭龍活血通瘀膠囊
고혈압경동맥죽양경화%NF-κB%ICAM-1%질룡활혈통어효낭
Hypertensive carotid atherosclerosis%NF-κB%ICAM-1%Zhilong Huoxue Tongyu capsul
目的：观察益气祛风通络法对高血压颈动脉粥样硬化家兔NF-κB与ICAM-1表达的影响，探讨益气祛风通络法减轻家兔高血压颈动脉粥样硬化的可能机制。方法：采用双侧肾动脉狭窄联合免疫损伤复合高脂饲料法建立高血压颈动脉粥样硬化家兔模型。将家兔随机分为假手术组、模型组、蛭龙活血通瘀胶囊低、中、高剂量组、辛伐他汀组。采用免疫组化法测定NF-κB与ICAM-1的表达。结果：模型组家兔颈动脉NF-κB、ICAM-1表达明显多于假手术组，差异有统计学意义（P＜0.05）；与模型组相比较，高、中剂量组及辛伐他汀组家兔颈动脉NF-κB、ICAM-1表达均明显减少，差异有统计学意义（P＜0.05）；高剂量组及辛伐他汀组比较差异无统计学意义（P>0.05）。结论：益气祛风通络法能明显减轻高血压颈动脉粥样硬化模型家兔颈动脉AS病变，其机制可能与抑制颈动脉组织NF-κB、ICAM-1的表达从而干预炎症反应有关。
目的：觀察益氣祛風通絡法對高血壓頸動脈粥樣硬化傢兔NF-κB與ICAM-1錶達的影響，探討益氣祛風通絡法減輕傢兔高血壓頸動脈粥樣硬化的可能機製。方法：採用雙側腎動脈狹窄聯閤免疫損傷複閤高脂飼料法建立高血壓頸動脈粥樣硬化傢兔模型。將傢兔隨機分為假手術組、模型組、蛭龍活血通瘀膠囊低、中、高劑量組、辛伐他汀組。採用免疫組化法測定NF-κB與ICAM-1的錶達。結果：模型組傢兔頸動脈NF-κB、ICAM-1錶達明顯多于假手術組，差異有統計學意義（P＜0.05）；與模型組相比較，高、中劑量組及辛伐他汀組傢兔頸動脈NF-κB、ICAM-1錶達均明顯減少，差異有統計學意義（P＜0.05）；高劑量組及辛伐他汀組比較差異無統計學意義（P>0.05）。結論：益氣祛風通絡法能明顯減輕高血壓頸動脈粥樣硬化模型傢兔頸動脈AS病變，其機製可能與抑製頸動脈組織NF-κB、ICAM-1的錶達從而榦預炎癥反應有關。
목적：관찰익기거풍통락법대고혈압경동맥죽양경화가토NF-κB여ICAM-1표체적영향，탐토익기거풍통락법감경가토고혈압경동맥죽양경화적가능궤제。방법：채용쌍측신동맥협착연합면역손상복합고지사료법건립고혈압경동맥죽양경화가토모형。장가토수궤분위가수술조、모형조、질룡활혈통어효낭저、중、고제량조、신벌타정조。채용면역조화법측정NF-κB여ICAM-1적표체。결과：모형조가토경동맥NF-κB、ICAM-1표체명현다우가수술조，차이유통계학의의（P＜0.05）；여모형조상비교，고、중제량조급신벌타정조가토경동맥NF-κB、ICAM-1표체균명현감소，차이유통계학의의（P＜0.05）；고제량조급신벌타정조비교차이무통계학의의（P>0.05）。결론：익기거풍통락법능명현감경고혈압경동맥죽양경화모형가토경동맥AS병변，기궤제가능여억제경동맥조직NF-κB、ICAM-1적표체종이간예염증반응유관。
Objective: To observe the therapeutic effect of Yiqi Qufeng Tongluo on expression of NF-κB and ICAM-1 in hypertensive carotid atherosclerosis, and its possible mechanism. Methods: Bilateral renal artery stenosis combined immunization injury and composite high fat feeding method was used to establish hypertensive carotid atherosclerosis model in rabbits. The rabbits were randomly divided into sham operation group, model group, Zhilong Huoxue Tongyu capsule low, middle, and high dose groups, and simvastatin group.Immunohisto chemical method was used to observe the expression of NF-κB and ICAM-1. Results:Rabbit carotid NF-κB and ICAM-1 were significantly higher in the model group than in the sham operation group,and the difference was statistically significant (P<0.05);Compared with the model group, rabbit carotid NF-κB and ICAM-1 in high, middle dose group and simvastatin group were decreased, and the difference was statistically significant (P<0.05). There was no significant difference between the high dose group and the simvastatin group (P>0.05). Conclusion:Yiqi Qufeng Tongluo therapy can obviously relieve AS lesions in the rabbit models, and its mechanism may be related to inhibition of the expression of NF-κB and ICAM-1 in carotic artery, thus intervening its inflammatory reaction.
