CAJ | 학술논문

目的：探讨人型支原体对氟喹诺酮类药物的耐药机制。方法自临床分离的8株耐氟喹诺酮类药物的人型支原体(Mh)，对其ParE基因PCR扩增后进行测序分析，与基因库中的野生型菌株MHPG21基因序列比对，分析ParE基因突变位点与菌株耐氟喹诺酮类药物的关系。结果与野生株MHPG21对比，6株检出ParE基因所编码的氨基酸残基发生D 426→N变异。结论 Mh临床分离株对氟喹诺酮类药物的耐药可能与ParE基因所编码的氨基酸残基D 426→N变异有关。
목적：탐토인형지원체대불규낙동류약물적내약궤제。방법자림상분리적8주내불규낙동류약물적인형지원체(Mh)，대기ParE기인PCR확증후진행측서분석，여기인고중적야생형균주MHPG21기인서렬비대，분석ParE기인돌변위점여균주내불규낙동류약물적관계。결과여야생주MHPG21대비，6주검출ParE기인소편마적안기산잔기발생D 426→N변이。결론 Mh림상분리주대불규낙동류약물적내약가능여ParE기인소편마적안기산잔기D 426→N변이유관。
Objective To explore the fluoroquinolones resistance meachanisms of Mycoplasma hominis (Mh). Methods A total of 8 strains of Mh with different fluoroquinolones resistance phenotype were analyzed, the ParE gene was amplified and sequenced. The sequencing results of ParE gene were compared with that in the wild type strain PG21, then analyzed the relation between ParE gene mutation and fluoroquinolones resistance. Results Compared with wild strain PG21, ParE gene in 6 strains got a D426→N mutation. Conclusion The fluoroquinolones resistance of Mh might be associate with ParE gene D426→N mutation.