CAJ | 학술논문

从粳稻品种 Asominori 的组培后代中发现了一个温度敏感的叶绿素缺乏突变体.低温(23℃)条件下该突变体幼苗3叶期前表现为白化表型随后致死,但在正常温度条件下与野生型无明显差异(30℃).与野生型相比,该突变体幼苗低温条件下叶绿素含量明显下降,叶绿体结构发育异常.遗传分析结果表明,该突变体受一对隐性核基因控制,定名为 cde2(chlo-rophyll deficient 2)基因.从 cde2与籼稻品种培矮64衍生的 F2群体中挑选1064株表现为突变表型的单株进行基因定位,将该基因初步定位于水稻第1染色体的着丝粒附近,随后利用已有的 SSR 标记和自行开发的 Indel 标记,进一步将该基因定位在标记 RM11041和 Indel1之间,物理距离为365．6 kb.此外,对该突变体叶绿素合成、光合作用以及质体转录/翻译系统相关基因的表达量测定表明,CDE2突变后增加了与叶绿素合成和质体转录/翻译相关基因的表达,但降低了光合作用相关基因的表达.结果表明,CDE2在水稻叶绿素合成以及叶绿体的发育过程中起着重要的作用.
종갱도품충 Asominori 적조배후대중발현료일개온도민감적협록소결핍돌변체.저온(23℃)조건하해돌변체유묘3협기전표현위백화표형수후치사,단재정상온도조건하여야생형무명현차이(30℃).여야생형상비,해돌변체유묘저온조건하협록소함량명현하강,협록체결구발육이상.유전분석결과표명,해돌변체수일대은성핵기인공제,정명위 cde2(chlo-rophyll deficient 2)기인.종 cde2여선도품충배왜64연생적 F2군체중도선1064주표현위돌변표형적단주진행기인정위,장해기인초보정위우수도제1염색체적착사립부근,수후이용이유적 SSR 표기화자행개발적 Indel 표기,진일보장해기인정위재표기 RM11041화 Indel1지간,물리거리위365．6 kb.차외,대해돌변체협록소합성、광합작용이급질체전록/번역계통상관기인적표체량측정표명,CDE2돌변후증가료여협록소합성화질체전록/번역상관기인적표체,단강저료광합작용상관기인적표체.결과표명,CDE2재수도협록소합성이급협록체적발육과정중기착중요적작용.
A thermo-sensitive chlorophyll deficient mutant was obtained from the progeny of tissue culture of a japonica rice cultivar Asominori.The mutant was characterized by albino leaves before 3-leaf stage under low temperature (23℃),while showing normal phenotype under high temperature(30℃).The chlorophyll contents of the mutant were significantly lower than those of wild-type before 3-leaf stage under low temperature,which is due to altered chloroplast development under low temperature.Genetic analysis showed that the mutation was controlled by a single recessive gene,which is tentatively designed as cde2 (chlorophyll deficient 2 ).Furthermore,using an F2 population derived from cde2/Peiai 64 and polymorphic markers between cde2 and Peiai 64,the cde2 was finally localized on the centromeric region of chromosome 1 and positioned between SSR markers RM1 1041 and Indel1 ,within the physical distance about 365 .6 kb.In addition, the expression level of genes associated with chlorophyll biosynthesis, photosynthesis and plastidial transcription/translation apparatus was measured and the results shows that the cde2 mutation increased the transcript level of chlorophyll biosynthesis genes and the platidial transcription/translation apparatus related genes,but reduced the expression level of photosynthesis genes,indicating that CDE2 may involve in the chlorophyll biosynthesis and chloroplast development.